Promotion of gastrulation by maternal growth factor in cultured rabbit blastocysts

Cell and Tissue Research
M Hrabé de AngelisC Kirchner

Abstract

Rabbit blastocysts of day 6 post coitus were cultured in a chemically defined, protein-free medium for 24 h. Although the trophoblast continued to grow, the embryonic disc degenerated. Addition of basic fibroblast growth factor (FGF-2, of human recombinant or bovine origin, 10 ng/ml) to the culture medium resulted in significant developmental progress. The embryonic disc became pear-shaped showing a round anterior edge and a posterior node. The primitive streak and Hensen's node indicated that gastrulation had begun. Mesoderm formation was confirmed from histological sections and by localization of the expression of T-gene transcripts in whole-mount preparations. FGF-2 mRNA was detected in both day-6 endometrium and day 6-blastocysts using in vitro translation followed by immunoprecipitation with a monoclonal antibody to FGF-2. In the uterine secretions of day-6 pregnant and pseudopregnant animals, several proteins exhibiting FGF-2 antigenicity were detected on Western blots following two-dimensional gel electrophoresis. As day-6 blastocysts required exogenous FGF-2 in vitro and as FGF-2 of uterine origin is present in the uterine secretion, the maternal growth factor can promote gastrulation in vivo.

References

Aug 1, 1976·European Journal of Biochemistry·H R Pelham, R J Jackson
Jan 24, 1992·Cell·T M Jessell, D A Melton
Sep 16, 1991·Biochemical and Biophysical Research Communications·R EinspanierK H Scheit
Jan 1, 1991·Anatomy and Embryology·D R DannhornC Kirchner
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H PratsJ A Smith
Mar 1, 1989·Journal of Reproduction and Fertility·D R BrigstockK D Brown
Jan 1, 1986·Cold Spring Harbor Symposia on Quantitative Biology·J A AbrahamJ C Fiddes
Sep 1, 1986·Journal of Cellular Physiology·D Gospodarowicz, J Cheng
Jul 1, 1987·Cell Differentiation·J BornH Tiedemann
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·F EschR Guillemin
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Aug 1, 1970·Journal of Reproduction and Fertility·J A Cholewa, W K Whitten
Feb 18, 1982·Nature·E M De RobertisR F Parisot
Aug 1, 1995·Anatomy and Embryology·C ViebahnM Hrabé de Angelis
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B C PariaS K Dey
Mar 1, 1995·Journal of Cellular Physiology·R Z FlorkiewiczE Florkiewicz
Jun 29, 1993·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J PartanenK Alitalo
Oct 1, 1993·Biology of Reproduction·D L Carlone, V Rider
May 1, 1993·Trends in Genetics : TIG·B Rosen, R S Beddington
Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S KlugD Neubert
Apr 1, 1993·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Elizabeth A JonesHugh R Woodland

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Citations

Nov 11, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Flavia N T CookeAlan D Ealy
Feb 21, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·L A OkumuP Lonergan
Jan 16, 2016·Growth Factors·Mehdi MoradiMohammad Hossein Nasr-Esfahani
Jul 27, 2001·Mechanisms of Development·S MildeC Kirchner
Mar 14, 2017·Reproductive Sciences·Gulcin Sahin ErsoyHugh S Taylor

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