Promotion of interferon-gamma production by natural killer cells via suppression of murine peritoneal macrophage prostaglandin E₂ production using intravenous anesthetic propofol

International Immunopharmacology
Takefumi InadaKoh Shingu

Abstract

Propofol is an intravenous anesthetic, widely used for general anesthesia during surgery, which inevitably involves tissue trauma with inflammation. At sites of inflammation, prostanoids, especially prostaglandin E₂ (PGE₂), are abundant. This study addresses the effect of propofol on macrophage PGE₂ production. Using thioglycollate-elicited murine peritoneal macrophages, propofol (7.5-30 μM) suppressed lipopolysaccharide-induced PGE₂ production. The suppression was via the direct inhibition of cyclooxygenase (COX) enzyme activity and due neither to the downregulation of COX expression nor the inhibition of arachidonic acid release from plasma membranes. In macrophage:natural killer (NK) cell co-culture, propofol dramatically increased interferon-gamma (IFN-γ) production, and the actions of propofol were mimicked by a selective COX-2 inhibitor, NS-398, as well as the selective EP4 receptor antagonist L-161,982, suggesting a role of PGE₂ suppression in the upregulation of IFN-γ production. Furthermore, in purified NK cell culture, PGE₂ directly suppressed the production of IFN-γ by activated NK cells, which was reversed by selective inhibition of EP4 activity. Taken together, our results show that, in macrophage:NK cell co-cultur...Continue Reading

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Citations

May 26, 2011·Journal of Anesthesia·Takefumi InadaKoh Shingu
Jun 26, 2014·Veterinary Anaesthesia and Analgesia·Stacy L AndersonBaljit Singh
Nov 1, 2011·Journal of Immunotoxicology·Takefumi InadaKoh Shingu
Jun 21, 2016·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Daniel A CaroffMichael Klompas
Dec 12, 2018·Anesthesiology·Daniel I Sessler, Bernhard Riedel
Aug 9, 2020·Local and Regional Anesthesia·Juan P CataMaria F Ramirez
Nov 19, 2020·Neuro-oncology Advances·Ann PrivorotskiyJuan P Cata
Mar 7, 2021·Research in Veterinary Science·Ciara A BarrOliver A Garden
Oct 17, 2021·Fundamental & Clinical Pharmacology·Priyanka SahaDona Sinha
Feb 20, 2020·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·Bon-Wook KooHyo-Seok Na

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