Abstract
Propofol is an intravenous anesthetic, widely used for general anesthesia during surgery, which inevitably involves tissue trauma with inflammation. At sites of inflammation, prostanoids, especially prostaglandin E₂ (PGE₂), are abundant. This study addresses the effect of propofol on macrophage PGE₂ production. Using thioglycollate-elicited murine peritoneal macrophages, propofol (7.5-30 μM) suppressed lipopolysaccharide-induced PGE₂ production. The suppression was via the direct inhibition of cyclooxygenase (COX) enzyme activity and due neither to the downregulation of COX expression nor the inhibition of arachidonic acid release from plasma membranes. In macrophage:natural killer (NK) cell co-culture, propofol dramatically increased interferon-gamma (IFN-γ) production, and the actions of propofol were mimicked by a selective COX-2 inhibitor, NS-398, as well as the selective EP4 receptor antagonist L-161,982, suggesting a role of PGE₂ suppression in the upregulation of IFN-γ production. Furthermore, in purified NK cell culture, PGE₂ directly suppressed the production of IFN-γ by activated NK cells, which was reversed by selective inhibition of EP4 activity. Taken together, our results show that, in macrophage:NK cell co-cultur...Continue Reading
References
Oct 1, 1995·Annals of Surgery·S T O'SullivanM L Rodrick
Feb 1, 1995·The Journal of Experimental Medicine·T C van der Pouw KraanL A Aarden
Nov 27, 1995·Biochemical Pharmacology·M T WithnallD Wiseman
Mar 1, 1996·Surgery·D DeckerA A von Ruecker
Mar 1, 1997·British Journal of Anaesthesia·S KahramanK Erdem
Nov 20, 1998·Anesthesiology·B LarsenM Bauer
Aug 31, 2000·Annual Review of Biochemistry·W L SmithR M Garavito
Sep 27, 2000·Proceedings of the National Academy of Sciences of the United States of America·Q JiangB N Ames
Jul 4, 2001·The Journal of Clinical Investigation·S L TilleyB H Koller
Aug 11, 2001·Der Anaesthesist·G HoffM Bauer
Feb 28, 2002·Trends in Immunology·Sarah G HarrisRichard P Phipps
Apr 11, 2002·Acta Anaesthesiologica Scandinavica·M TakaonoS Aotsuka
Jun 5, 2003·International Immunopharmacology·Szczepan JózefowskiJanusz Marcinkiewicz
Jul 10, 2003·Archives of Toxicology·Ruei-Ming ChenTa-Liang Chen
Feb 12, 2004·The Journal of Pharmacology and Experimental Therapeutics·Shinichiro KuboMasahiro Nishibori
Mar 11, 2004·Immunology·William Walker, Dino Rotondo
Aug 20, 2004·Pharmacological Reviews·Daniel L SimmonsTimothy Hla
Sep 7, 2004·British Journal of Anaesthesia·J R Sneyd
May 19, 2005·Pharmacotherapy·Paul E Marik
Jun 21, 2005·Annals of the New York Academy of Sciences·Ruei-Ming ChenChih-Hsiung Wu
Jun 30, 2005·European Journal of Pharmacology·Hideo K TakahashiMasahiro Nishibori
Jul 11, 2006·Immunology·Mitsuhiro OkanoKazunori Nishizaki
Mar 27, 2007·European Journal of Pharmacology·Jung-Yeon YunZhiyi Zuo
Jul 21, 2007·Acta Pharmacologica Sinica·Xing-jun WuHong-zhuan Chen
Dec 8, 2007·Naunyn-Schmiedeberg's Archives of Pharmacology·J A González-CorreaJ P De La Cruz
Mar 20, 2008·Anesthesia and Analgesia·Agnes RigouzzoIsabelle Constant
May 24, 2008·Anesthesiology·Viviana CavalcaErminio Sisillo
Aug 30, 2008·Biochemical Pharmacology·Wen LinAh-Ng Kong
Feb 28, 2009·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Takefumi InadaKoh Shingu
Feb 28, 2009·European Journal of Pharmacology·Ioanna VasileiouLila Papadimitriou
Mar 11, 2009·The Journal of Experimental Medicine·Katia BonifaceRené de Waal Malefyt
May 26, 2009·Nature Medicine·Chengcan YaoShuh Narumiya
Dec 3, 2009·Immunopharmacology and Immunotoxicology·Tomoko KambaraKoh Shingu
Citations
May 26, 2011·Journal of Anesthesia·Takefumi InadaKoh Shingu
Apr 23, 2014·Journal of Anesthesia·Ju Deok KimSoo Bong Yu
Jun 26, 2014·Veterinary Anaesthesia and Analgesia·Stacy L AndersonBaljit Singh
Jul 22, 2014·Journal of Immunotoxicology·Takefumi InadaKoh Shingu
Nov 1, 2011·Journal of Immunotoxicology·Takefumi InadaKoh Shingu
Jun 21, 2016·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Daniel A CaroffMichael Klompas
Apr 12, 2016·Korean journal of anesthesiology·Ji Heui LeeBong Seog Kim
Sep 17, 2011·Shock·Huang-Ping Yu
Dec 12, 2018·Anesthesiology·Daniel I Sessler, Bernhard Riedel
Sep 2, 2020·Biology·George AndersonGianluigi Mazzoccoli
Sep 24, 2018·BMC Anesthesiology·Tao YanHui Zheng
Aug 9, 2020·Local and Regional Anesthesia·Juan P CataMaria F Ramirez
Nov 22, 2013·The Annals of Pharmacotherapy·Michael A SmithKerry M Empey
Nov 19, 2020·Neuro-oncology Advances·Ann PrivorotskiyJuan P Cata
Nov 24, 2020·Annals of Medicine and Surgery·Ryungsa KimKoji Arihiro
Mar 7, 2021·Research in Veterinary Science·Ciara A BarrOliver A Garden
Sep 22, 2020·Urologic Oncology·John L PfailJohn P Sfakianos
Oct 17, 2021·Fundamental & Clinical Pharmacology·Priyanka SahaDona Sinha
Feb 20, 2020·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·Bon-Wook KooHyo-Seok Na