Promotion of neurite outgrowth by rationally designed NGF-β binding peptide nanofibers

Biomaterials Science
Zeynep OkurAyse B Tekinay

Abstract

Promotion of neurite outgrowth is an important limiting step for regeneration in nerve injury and depends strongly on the local expression of nerve growth factor (NGF). The rational design of bioactive materials is a promising approach for the development of novel therapeutic methods for nerve regeneration, and biomaterials capable of presenting NGF to nerve cells are especially suitable for this purpose. In this study, we show bioactive peptide amphiphile (PA) nanofibers capable of promoting neurite outgrowth by displaying high density binding epitopes for NGF. A high-affinity NGF-binding sequence was identified by phage display and combined with a beta-sheet forming motif to produce a self-assembling PA molecule. The bioactive nanofiber had higher affinity for NGF compared to control nanofibers and in vitro studies revealed that the NGF binding peptide amphiphile nanofibers (NGFB-PA nanofiber) significantly promote the neurite outgrowth of PC-12 cells. In addition, the nanofibers induced differentiation of PC-12 cells into neuron-like cells by enhancing NGF/high-activity NGF receptor (TrkA) interactions and activating MAPK pathway elements. The NGFB-PA nanofiber was further shown as a promising material to support axonal outg...Continue Reading

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Citations

Jul 28, 2019·Macromolecular Rapid Communications·Priyadarshi ChakrabortyEhud Gazit
Aug 11, 2020·Reviews in the Neurosciences·Hamidreza ArzaghiHamidreza Pazoki-Toroudi
May 10, 2021·Advanced Drug Delivery Reviews·Christina Karavasili, Dimitrios G Fatouros
Jul 28, 2019·Journal of Medicinal Chemistry·Kristel C TjandraJoshua A McCarroll
Jul 7, 2018·ACS Applied Materials & Interfaces·Adam D MartinLars M Ittner
Nov 21, 2020·Chemical Reviews·Febrizio GelainShuguang Zhang

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Methods Mentioned

BETA
phage display
Assay
ELISA
scanning electron microscopy
confocal microscopy
electrophoresis

Software Mentioned

ImageJ

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