Promotion of regeneration and axon growth following injury in an invertebrate nervous system by the use of three-dimensional collagen gels

Proceedings. Biological Sciences
S E BlackshawJ A Davies

Abstract

We describe the application of three-dimensional collagen matrices to the study of nerve cord repair in the leech. Our experiments show that ganglia and connectives of the leech ventral nerve cord can be maintained for up to four weeks embedded in 3D gels constructed from mammalian type I collagen. Severed nerve cords embedded in the collagen gel reliably repaired within a few days of culture. The gel was penetrable by cells emigrating from the cut ends of nerves and connectives, and we consistently saw regenerative outgrowth of severed peripheral and central axons into the gel matrix. Thus, 3D gels provide an in vitro system in which we can reliably obtain repair of severed nerve cords in the dish, and visualize cell behaviour underlying regenerative growth at the damage site: and which offers the possibility of manipulating the regenerating cells and their extracellular environment in various ways at stages during repair. Using this system it should be possible to test the effect on the repair process of altering expression of selected genes in identified nerve cells.

References

Nov 12, 1976·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·K J Muller, U J McMahan
Jun 22, 1991·Proceedings. Biological Sciences·B StolzD P Kuffler
Jan 1, 1971·The Journal of Comparative Neurology·R S Tulsi, R E Coggeshall
Aug 1, 1983·Brain Research·V J MorgeseK J Muller
Mar 1, 1981·The Journal of Cell Biology·H K KleinmanG R Martin
Jan 1, 1982·Differentiation; Research in Biological Diversity·D FlynnS Nandi
Dec 1, 1981·The Journal of Cell Biology·R J ManninoM M Burger
Jul 1, 1995·Journal of Neurobiology·R von Bernhardi, K J Muller
Jul 1, 1995·Journal of Neurobiology·F Fernández-de-Miguel, P Drapeau
Feb 1, 1994·Progress in Neurobiology·S KorneevJ A Davies
Jan 22, 1996·Proceedings. Biological Sciences·S KorneevJ A Davies

❮ Previous
Next ❯

Citations

Sep 21, 2005·Invertebrate Neuroscience : in·E J BabingtonS E Blackshaw
Mar 3, 2006·Cell and Tissue Banking·Ewa Lesiak-CyganowskaJanusz Komender
Jul 29, 2005·Cellular and Molecular Neurobiology·W Z WangS E Blackshaw
Jan 22, 2004·Biochemical and Biophysical Research Communications·M W HaymanS A Przyborski
Feb 10, 2009·Tissue Engineering. Part a·Christian WeinandJoseph P Vacanti
Nov 26, 2008·Developmental Neurobiology·Yuanli DuanKenneth J Muller
Mar 1, 2005·Journal of Biochemical and Biophysical Methods·M W HaymanS A Przyborski
Apr 25, 2017·Tissue Engineering. Part a·Merav Antman-PassigOrit Shefi
Jun 1, 2017·Biotechnology Advances·Sheva NaahidiP Chen
Mar 20, 2018·International Journal of Biomaterials·Magdalini TsintouAlexander Seifalian
May 8, 2007·The Journal of Comparative Neurology·Emmanuel Mbaku NguKenneth J Muller
Mar 8, 2016·Advanced Healthcare Materials·Manuela LoebleinEdwin Hang Tong Teo
Jul 28, 2010·Biochemical Society Transactions·Daniel J Maltman, Stefan A Przyborski

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.