Promotion of Vascular Morphogenesis of Endothelial Cells Co-Cultured with Human Adipose-Derived Mesenchymal Stem Cells Using Polycaprolactone/Gelatin Nanofibrous Scaffolds

Nanomaterials
Yun-Min KookWon-Gun Koh

Abstract

New blood vessel formation is essential for tissue regeneration to deliver oxygen and nutrients and to maintain tissue metabolism. In the field of tissue engineering, in vitro fabrication of new artificial vessels has been a longstanding challenge. Here we developed a technique to reconstruct a microvascular system using a polycaprolactone (PCL)/gelatin nanofibrous structure and a co-culture system. Using a simple electrospinning process, we fabricated three-dimensional mesh scaffolds to support the sprouting of human umbilical vein endothelial cells (HUVECs) along the electrospun nanofiber. The co-culture with adipose-derived mesenchymal stem cells (ADSCs) supported greater sprouting of endothelial cells (ECs). In a two-dimensional culture system, angiogenic cell assembly produced more effective direct intercellular interactions and paracrine signaling from ADSCs to assist in the vascular formation of ECs, compared to the influence of growth factor. Although vascular endothelial growth factor and sphingosine-1-phosphate were present during the culture period, the presence of ADSCs was the most important factor for the construction of a cell-assembled structure in the two-dimensional culture system. On the contrary, HUVECs co-c...Continue Reading

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Citations

Aug 6, 2019·Connective Tissue Research·Betül Çelebi-SaltikBeyza Gökçinar-Yagci
Nov 8, 2018·International Journal of Molecular Sciences·Chiara ArgentatiSabata Martino
Dec 12, 2018·Frontiers in Physiology·Yulia A PaninaAlla B Salmina
Jul 17, 2020·Frontiers in Bioengineering and Biotechnology·Sneha GopalJonathan S Dordick
Dec 16, 2020·ACS Biomaterials Science & Engineering·Mina VardianiMazaher Gholipourmalekabadi

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Methods Mentioned

BETA
scanning electron microscopy
flow cytometry

Software Mentioned

SigmaPlot
ZEN

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