Prompt epithelial damage and restitution processes in allergen challenged guinea-pig trachea in vivo

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
J S ErjefältC G Persson

Abstract

Little is known about the induction and the morphology of epithelial damage, and of the ensuing epithelial restitution processes in allergic airways. To examine epithelial damage and restitution in allergen challenged guinea-pig trachea. Whole-mount techniques, transmission and scanning electron microscopy, cryosectioning, and histochemical staining were used. Cell proliferation was monitored by BrdU-immunohistochemistry. Allergen challenge produced patchy, crater-like, and leucocyte-rich epithelial damage sites. At 1, 5, and 24 h damage was associated with poorly differentiated epithelial restitution cells. Already at 1 h the epithelial craters had a floor of flattened restitution cells and the damaged areas comprised < 1% of the mucosal surface area (whole-mount preparations). In contrast, cryo sections displayed large areas (approximately 20%, 1 h) of denudation. Epithelial, and subepithelial (fibroblasts, smooth muscle) proliferation was increased 5 and 24 h after challenge (P < 0.01). Within 1 h allergen challenge has induced patchy damage sites where epithelial restitution is already advanced; although easily produced by cryosectioning frank denudation was not evident in whole-mount preparations. The present findings may ...Continue Reading

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Citations

Jan 20, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P M Lackie
Apr 24, 2012·Journal of Biomedicine & Biotechnology·John P SoleasThomas K Waddell
Sep 30, 2014·Laboratory Investigation; a Journal of Technical Methods and Pathology·Simon G RoyceChrishan S Samuel
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Oct 2, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J S ErjefältC G Persson
Dec 7, 2010·Expert Review of Respiratory Medicine·Darryl A KnightTillie-Louise Hackett
Dec 6, 2001·American Journal of Respiratory and Critical Care Medicine·P K Jeffery
Jul 16, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·F L M RicciardoloT Mauad
Jan 7, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J S ErjefältC G Persson
Jul 13, 2021·Frontiers in Physiology·François M CarlierCharles Pilette

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