Abstract
The amphibian pronephros is fated to die during early development. Pronephric cells undergo apoptosis and their function is replaced by the mesonephros, which becomes the functional kidney of the adult frog. Tadpoles of the northern leopard frog, Rana pipiens, were inoculated with a Lucké tumour herpesvirus (LTV) preparation. Most of the animals developed typical Lucké renal carcinomas at metamorphosis. Fewer developed carcinomas of the pronephric cell type. A pronephric carcinoma, rescued from apoptosis by the herpesvirus, was harvested from a post-metamorphic frog. The tumour was judged to be pronephric by its anatomical location (in the anterior part of the body) and because both mesonephric kidneys were intact and tumour-free upon removal of the tumour mass. A tumour fragment was fixed for histological examination, which confirmed that the tissue was a renal carcinoma. A further fragment was subjected to short-term culture in order to produce metaphase cells for cytogenetical analysis. Based upon silverstained nucleolar organizing region numbers, 14 of 15 metaphase cells were estimated to have the diploid number (2N = 26) of chromosomes and a karyotype was constructed which did not appear to differ from that of normal cells...Continue Reading
References
Mar 1, 1977·Stain Technology·D J Picciano, R G McKinnell
Jan 1, 1978·Experimental Cell Biology·R J Przybelski, K S Tweedell
Jul 25, 1991·Nature·E Yonish-RouachM Oren
May 1, 1990·Clinical & Experimental Metastasis·J W WilliamsR G McKinnell
Aug 1, 1972·British Journal of Cancer·J F KerrA R Currie
Aug 15, 1980·Experientia·W M Howell, D A Black
May 1, 1994·Journal of Comparative Pathology·D L CarlsonR G McKinnell
Sep 7, 1994·Journal of the National Cancer Institute·B W Stewart
Mar 1, 1994·British Journal of Cancer·A J LevineD Welsh
Oct 1, 1993·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·C Koseki
Jan 1, 1993·Cytogenetics and Cell Genetics·J W WilliamsR G McKinnell
Mar 1, 1963·The Quarterly Review of Biology·H FOX
Jun 26, 1964·Science·K WOLF, M C QUIMBY
May 1, 1952·Proceedings of the National Academy of Sciences of the United States of America·R Briggs, T J King
Sep 30, 1938·The Journal of Experimental Medicine·B Lucké
Citations
Dec 16, 1998·Lancet·N J Vogelzang, W M Stadler
Aug 15, 1997·Developmental Biology·P D VizeJ B Wallingford
Sep 12, 2001·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·S Essbauer, W Ahne