PMID: 3765337Jun 1, 1986Paper

Pronephric leucocytes of Cyprinus carpio: isolation, separation and characterization

Veterinary Immunology and Immunopathology
C J Bayne

Abstract

Since the teleost pronephros is an important source of diverse immunocytes, suspensions of pronephric cells from young adult carp have been characterized. In freshly prepared suspensions, adherent, spreading cells (macrophages?) constituted less than 3% of the total population. Granulocytes and lymphocytes were co-dominant (less than 80%) leucocyte types. Continuous Percoll density gradient centrifugation yielded discrete subpopulations with these rho values and cytological characteristics: Fraction I & II rho = 1.055-1.070 thrombocytes, monocytes, macrophages, and lymphocytes. Fraction III rho = 1.080-1.090 granulocytes, type 1. Fraction IV rho = 1.105-1.110 erythrocytes and granulocytes, type 2. Fraction V rho = 1.118-1.125 granulocytes, type 3. Fraction VI rho = 1.140-1.150 granulocytes, type 4. Granulocyte motility increased markedly over the first 24 hr in vitro, and was enhanced by components washed from intact yeast. The subtypes of granulocytes were distinguishable by not only the rho values, but also on the basis of cell size, ultra-structure of the granules, and their histochemical and phagocytic characteristics. After simultaneous in vivo injection of Bacillus megaterium (Gram + ve), Aeromonas hydrophila (Gram - ve) ...Continue Reading

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Citations

Jun 9, 2012·In Vitro Cellular & Developmental Biology. Animal·Dharmendra K ChaudharyGaurav Rathore
Jan 1, 1987·Developmental and Comparative Immunology·J H Temmink, C J Bayne
Jan 1, 1988·Developmental and Comparative Immunology·M Cossarini-DunierV Guerin
Jan 1, 1989·Developmental and Comparative Immunology·B PłytyczC J Bayne
Jan 1, 1990·Developmental and Comparative Immunology·J F SövényiR Kusuda
Jul 1, 1995·Developmental and Comparative Immunology·O KurataY Ikeda
Apr 1, 1992·Veterinary Immunology and Immunopathology·A Estepa, J M Coll
Jan 24, 2009·Photochemistry and Photobiology·Eveliina MarkkulaIlmari E Jokinen
May 4, 2007·Fish & Shellfish Immunology·Anita RønnesethEirin Fausa Pettersen
May 15, 2013·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Yen-Hua ChenSen-Shyong Jeng
Apr 22, 2003·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Alejandro RodríguezJosé Meseguer
Jun 1, 1988·The Journal of Experimental Zoology·B PłytyczC J Bayne
Dec 10, 2008·Developmental and Comparative Immunology·Patrick C HaningtonMiodrag Belosevic

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