Proof of Concept for MBT ASTRA, a Rapid Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS)-Based Method To Detect Caspofungin Resistance in Candida albicans and Candida glabrata

Journal of Clinical Microbiology
Mansoureh VatanshenassanKatrin Sparbier

Abstract

The incidence of candidemia caused by Candida albicans and Candida glabrata is constantly increasing and is accompanied by the rising use of the few available antifungals. The widespread use of echinocandins and azoles for the treatment of invasive candidemia has enhanced the development of antifungal resistance, resulting in an increasing health care problem. Hence, the rapid detection of resistant strains is required. This study aimed to evaluate the detection of C. albicans and C. glabrata strains resistant to caspofungin by the matrix-assisted laser desorption ionization Biotyper antibiotic susceptibility test rapid assay (MBT ASTRA). This novel semiquantitative technique facilitates the detection of caspofungin-resistant strains within 6 h. MBT ASTRA results were compared to the data obtained by the use of Clinical and Laboratory Standards Institute (CLSI) guidelines. Clinical isolates of C. albicans (n = 58) and C. glabrata (n = 57) were analyzed by MBT ASTRA and the CLSI microdilution method. Antifungal susceptibility testing against caspofungin by the CLSI microdilution method classified the C. albicans isolates into 36 susceptible and 22 resistant strains and the C. glabrata isolates into 5 susceptible, 33 resistant, a...Continue Reading

References

Jun 16, 2007·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·David S Perlin
Aug 19, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M Cuenca-EstrellaUNKNOWN AFST Subcommittee of EUCAST
Aug 31, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·F DrobniewskiUNKNOWN Subcommittee on Antimicrobial Susceptibility Testing of Mycobacterium tuberculosis of the European Committee for Antimicrobi
Feb 15, 2008·The Journal of Antimicrobial Chemotherapy·Elizabeth M Johnson
Aug 17, 2010·Current Opinion in Critical Care·Maiken C Arendrup
Nov 19, 2011·FEMS Microbiology Reviews·Antony CroxattoGilbert Greub
Jan 4, 2012·The American Journal of Medicine·Michael A Pfaller
Jul 17, 2012·Bioinformatics·Sebastian Gibb, Korbinian Strimmer
Nov 22, 2012·Expert Review of Clinical Immunology·Małgorzata MikulskaClaudio Viscoli
Jun 7, 2013·Expert Opinion on Drug Discovery·Christopher G Pierce, Jose L Lopez-Ribot
Sep 6, 2013·Journal of Clinical Microbiology·Katrin SparbierMarkus Kostrzewa
Oct 26, 2013·Virulence·Julie Delaloye, Thierry Calandra
Jun 1, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Nicholas D BeydaKevin W Garey
Oct 11, 2014·Future Microbiology·Brunella Posteraro, Maurizio Sanguinetti
Jan 26, 2016·Methods : a Companion to Methods in Enzymology·Katrin SparbierMarkus Kostrzewa
Jan 27, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Peter G PappasJack D Sobel
May 28, 2016·Critical Care : the Official Journal of the Critical Care Forum·Thierry CalandraJean-Louis Vincent
Jun 2, 2016·Annals of Clinical Microbiology and Antimicrobials·Hatice Turk DagiUgur Arslan
Oct 4, 2016·Future Microbiology·Elvira RománJesús Pla

❮ Previous
Next ❯

Citations

Jan 15, 2019·Expert Review of Molecular Diagnostics·Grégory DubourgFlorence Fenollar
Apr 24, 2020·Expert Review of Anti-infective Therapy·Ludwig Knabl, Cornelia Lass-Flörl
May 1, 2020·Clinical Microbiology Reviews·Elizabeth L BerkowLuis Ostrosky-Zeichner
Mar 6, 2019·Frontiers in Cellular and Infection Microbiology·Mansoureh VatanshenassanMarkus Kostrzewa
Feb 23, 2019·Frontiers in Cellular and Infection Microbiology·Margot DelavyAlix T Coste
Jan 31, 2019·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Belén Rodríguez-SánchezGilbert Greub
Oct 22, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sachio TsuchidaTomohiro Nakayama
Jan 15, 2021·Journal of Fungi·Lewis P White, Jessica S Price
Jun 4, 2019·FEMS Microbiology Reviews·UNKNOWN Consortium OPATHY, Toni Gabaldón
May 27, 2021·Studies in Mycology·A ArastehfarM Hoenigl
Jul 3, 2021·Microorganisms·Marie-Gladys RobertDanièle Maubon
Jun 17, 2021·Journal of Clinical Microbiology·Evgeny A Idelevich, Karsten Becker

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.