Proof of concept pilot study: prevalence of grass virus infection and the potential for effects on the allergenic potency of pollen.

Environmental Health : a Global Access Science Source
Denise PallettHui Wang

Abstract

Wild plants harbour a variety of viruses and these have the potential to alter the composition of pollen. The potential consequences of virus infection of grasses on pollen-induced allergic disease are not known. We have collected pollen from Dactylis glomerata (cocksfoot; a grass species implicated as a trigger of allergic rhino-conjunctivitis) from Wytham Wood, Oxfordshire UK. Extracts were prepared from pollen from uninfected grass, and from grass naturally infected by the Cocksfoot streak potyvirus (CSV). Preparations of pollen from virus-infected and non-infected grasses were employed in skin testing 15 grass pollen-allergic subjects with hayfever. Allergen profiles of extracts were investigated by Western blotting for IgE with sera from allergic subjects. The prevalence of CSV infection in cocksfoot grasses sampled from the study site varied significantly over an eight-year period, but infection rates of up to 70% were detected. Virus infection was associated with small alterations in the quantities of pollen proteins detected by polyacrylamide gel electrophoresis, and in the patterns of allergens identified by Western blotting with IgE from grass pollen allergic subjects. For individual subjects there were differences in...Continue Reading

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Citations

Sep 5, 2020·Clinical and Translational Allergy·Lisa PointnerLorenz Aglas

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Methods Mentioned

BETA
Enzyme-Linked ImmunoSorbent Assay
electrophoresis
light microscopy
ELISA

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