Proof of concept study of age-dependent DNA methylation markers across different tissues by massive parallel sequencing

Forensic Science International. Genetics
Jana NauePernette J Verschure

Abstract

The use of DNA methylation (DNAm) for chronological age determination has been widely investigated within the last few years for its application within the field of forensic genetics. The majority of forensic studies are based on blood, saliva, and buccal cell samples, respectively. Although these types of samples represent an extensive amount of traces found at a crime scene or are readily available from individuals, samples from other tissues can be relevant for forensic investigations. Age determination could be important for cases involving unidentifiable bodies and based on remaining soft tissue e.g. brain and muscle, or completely depend on hard tissue such as bone. However, due to the cell type specificity of DNAm, it is not evident whether cell type specific age-dependent CpG positions are also applicable for age determination in other cell types. Within this pilot study, we investigated whether 13 previously selected age-dependent loci based on whole blood analysis including amongst others ELOVL2, TRIM59, F5, and KLF14 also have predictive value in other forensically relevant tissues. Samples of brain, bone, muscle, buccal swabs, and whole blood of 29 deceased individuals (age range 0-87 years) were analyzed for these ...Continue Reading

Citations

Sep 7, 2019·Journal of Forensic Sciences·Helena Correia DiasLicínio Manco
Jan 4, 2020·International Journal of Legal Medicine·Ana Belén Márquez-RuizAurora Valenzuela
Jan 2, 2021·Scientific Reports·Yang HanWolfgang Wagner
Jan 20, 2021·Development and Psychopathology·Laura Ramo-FernándezIris-Tatjana Kolassa
Feb 23, 2020·Forensic Science International. Genetics·Hwan Young LeeYang Han Lee
Feb 26, 2021·Electrophoresis·Ting HaoGengqian Zhang
Apr 28, 2020·Computers in Biology and Medicine·Najmeh Sadat Jaddi, Mohammad Saniee Abadeh
May 1, 2021·International Journal of Molecular Sciences·Sara C ZapicoBruce R McCord
Jul 13, 2021·Frontiers in Genetics·Cemal GurkanKenneth K Kidd
Nov 30, 2018·Analytical Chemistry·Bruce R McCordGeorge Duncan

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