Proof-of-concept study to establish an in situ method to determine the nature and depth of collagen changes in dentine using Fourier Transform Infra-Red spectroscopy after sodium hypochlorite irrigation

International Endodontic Journal
A D MorganL Bozec

Abstract

To establish a method using Fourier Transform Infra-Red spectroscopy (FTIR) to characterize the nature and depth of changes in dentinal collagen following exposure to sodium hypochlorite (NaOCl) during root canal irrigation in an ex vivo model. Fourier Transform Infra-Red spectroscopy was used to assess the changes in dentinal collagen when the root canal was exposed to NaOCl. The changes in dentinal collagen caused by NaOCl irrigation of root canals in transverse sections of roots, at 0.5 mm from the canal wall and 0.5 mm from the external root surface, were assessed by FTIR. The data were analysed using paired t-test with 5% significance level. Fourier Transform Infra-Red spectroscopy confirmed that NaOCl exposure caused alterations in the chemistry and structure of collagen in dentine. FTIR spectra obtained from dentine surfaces and dentine adjacent to root canals exposed to NaOCl, all consistently showed degradation and conformational change of the collagen structure. FTIR data from the ex vivo model showed that the depth of effect of NaOCl extended to at least 0.5 mm from the canal wall. In extracted human teeth, NaOCl caused changes in dentinal collagen that were measurable by FTIR. In an ex vivo model, the depth of effec...Continue Reading

References

Sep 1, 1988·Journal of Dental Research·T SakaeY Kozawa
Nov 1, 1994·International Endodontic Journal·S V BarbosaS W Spångberg
Jul 1, 1993·Dental Materials : Official Publication of the Academy of Dental Materials·G W MarshallS J Marshall
Feb 1, 1993·Journal of Biomedical Materials Research·B B TomazicE D Eanes
Apr 1, 1996·European Journal of Oral Sciences·H TsudaJ Arends
May 30, 1998·Journal of Dentistry·G W MarshallM Balooch
Sep 28, 2000·Micron : the International Research and Review Journal for Microscopy·V OttaniA Ruggeri
Apr 20, 2001·International Endodontic Journal·T P SimK Gulabivala
Oct 5, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·E P PaschalisM Yamauchi
Oct 31, 2001·Calcified Tissue International·J H KinneyS J Marshall
Jun 8, 2002·Journal of Biomechanics·Stefan HabelitzSally J Marshall
Sep 20, 2002·Brazilian Dental Journal·Carlos EstrelaJesus D Pécora
Jan 30, 2004·Journal of Dentistry·Bor-Shiunn LeeChun-Pin Lin
May 12, 2004·Dental Materials : Official Publication of the Academy of Dental Materials·Victoria FuentesDavid H Pashley
Sep 7, 2004·Journal of Materials Science. Materials in Medicine·C O DriscollK Gulabivala
Nov 27, 2004·Journal of Endodontics·Iris Slutzky-GoldbergIlana Heling
Aug 19, 2007·International Endodontic Journal·M MarendingM Zehnder
Aug 22, 2007·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Taner Cem SayinHarika G Otlu
May 10, 2008·Journal of Structural Biology·M BaloochG W Marshall
Jul 1, 2008·Journal of Dentistry·Ana Flávia Sanches BorgesRegina Maria Puppin Rontani
Feb 26, 2010·Dental Materials : Official Publication of the Academy of Dental Materials·Kai ZhangJun-qi Ling
Apr 20, 2010·Biomaterials·Anke MärtenPaul Zaslansky
May 9, 2012·Beilstein Journal of Nanotechnology·Sergiu AmarieFritz Keilmann
Apr 16, 2014·Dental Materials : Official Publication of the Academy of Dental Materials·Ksenija ZelicMarija Djuric
Jul 8, 2016·Journal of Applied Oral Science : Revista FOB·Talita TartariClovis Monteiro Bramante

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Citations

May 28, 2019·British Dental Journal·Kishor Gulabivala, Yuan-Ling Ng

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