Propagated and nonpropagated calcium transients during egg activation in the annelid, Chaetopterus

Developmental Biology
W R Eckberg, A L Miller

Abstract

Transient waves of Ca2+ release cross-fertilizing deuterostome eggs from the point of sperm entry to its antipode and provide much of the activating stimulus for the egg. Based on several indirect lines of experimental evidence, it was proposed that protostome eggs are activated by a prolonged uptake of Ca2+ from the medium due to sperm-induced membrane depolarization and that this uptake then starts an activation wave similar to those in deuterostomes, except that it moves inward from the whole surface rather than through the egg from pole to pole. To test these hypotheses, we microinjected oocytes of the polychaete annelid, Chaetopterus pergamentaceus, with semisynthetic recombinant aequorins and measured light emission in response to both fertilization and artificial activation by excess K+. Both fertilization and K(+)-activation induced multiple, brief Ca2+ transients in the eggs. The first transient did not propagate, but it was followed by a series of globally propagated Ca2+ waves interspersed with additional nonpropagated pulses. The waves traversed the egg at about 30 micrometer/sec. Sequential propagated waves and nonpropagated pulses generally originated at different regions of the egg surface, except the last few, w...Continue Reading

Citations

Oct 16, 2002·Cell Calcium·L Jaffe
Oct 13, 2006·Biophysical Chemistry·C SardetA McDougall
Feb 12, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Michael Whitaker
Jan 7, 2004·Development, Growth & Differentiation·Shantá D HintonWilliam R Eckberg
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·E GillandS E Webb
Jun 24, 2014·Biochemical and Biophysical Research Communications·Takeshi NakanoKeiichiro Kyozuka
Oct 5, 2010·Cell Calcium·Lionel F Jaffe
Aug 1, 2013·Molecular Reproduction and Development·Junaid KashirStephen A Stricker
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