Jun 10, 2004

Propagation and maintenance of undifferentiated human embryonic stem cells

Stem Cells and Development
Megan S BodnarMeri T Firpo


Human embryonic stem (hES) cells, like other stem cells, have the capacity to self-renew without differentiation. Although hES cells can be differentiated to many different tissue types in vitro, clinical uses have not yet been realized from the study of hES cells. Anticipation that these cells would be immediately useful for creating models of human disease has not yet been fulfilled. However, because of their self-renewing and pluripotential nature, hES cells indeed hold unique promise for many areas of research and medicine. A major problem complicating developments in hES cell research is the difficulty of propagating and maintaining these cells in vitro without differentiation. This review addresses this problem and potential solutions in detail. In addition, the current state of research regarding the growth and maintenance of hES cells is summarized, along with basic protocols utilized by our laboratory for the successful propagation, characterization, and investigation of hES cells.

  • References44
  • Citations35


  • References44
  • Citations35

Mentioned in this Paper

Cell Culture Techniques
Histocompatibility Testing
Gene Expression
Cell Differentiation Process
Mature Teratoma
Colony-forming Unit
Embryonic Stem Cells
In Vitro [Publication Type]

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