PMID: 8584217Oct 13, 1995Paper

Propagation of intercellular calcium waves in PC12 cells overexpressing a carboxy-terminal fragment of amyloid precursor protein

Neuroscience Letters
B D LynnJ I Nagy

Abstract

We have recently found that PC12 cells overexpressing a C-terminal 97 amino acid fragment containing the beta/A4 amyloid peptide of amyloid precursor protein (APP) exhibit an induced production of the gap junctional protein connexin43 (Cx43) and an induction of gap junctional communication as assessed by dye-transfer. In studies of two beta/A4-transfected PC12 clones, we show here that these cells, unlike normal PC12 cells or those transfected with vector alone, have the capacity for intercellular transmission of calcium waves as determined by imaging of calcium with fura-2. Intercellular wave propagation occurred in the absence of extracellular calcium and was blocked by known inhibitors of gap junctional communication (octanol and 12-O-tetradecanoyl-phorbol-13-acetate), suggesting mediation by gap junctions. The results indicate a disruptive influence of the C-terminal region of APP or of beta/A4 amyloid peptide on intercellular signaling via gap junctions, which may be relevant to the normal functions of APP or to pathology in Alzheimer's disease.

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Citations

Mar 23, 2012·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Eliseo A EugeninJoan W Berman
Aug 12, 2009·Neurotoxicity Research·Ronald L KleinMichael A King
Apr 7, 2000·Brain Research. Brain Research Reviews·J I Nagy, J E Rash
Oct 23, 1997·The Journal of Cell Biology·N R JorgensenT H Steinberg
Nov 23, 2005·Journal of Neuroscience Research·Paul CushingDaniel J Belliveau
May 30, 2006·The Journal of Biological Chemistry·Daniel J BelliveauWalter J Rushlow
Jul 20, 2012·Physiological Reviews·Luc Leybaert, Michael J Sanderson

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