PMID: 7540969Jan 1, 1994Paper

Propagation of mouse and human T cells with defined antigen specificity and function

Ciba Foundation Symposium
P A CohenS A Rosenberg

Abstract

Difficulties maintaining fully functional CD4+ T cells in culture have historically limited the study of their role in tumour rejection as well as other clinical applications. As the therapeutic value of current antitumour CD8+ T cell adoptive therapy becomes better defined, a strong impetus exists to determine optimal conditions for culturing antitumour CD4+ T cells. Our goal is to promote broadly polyclonal, antigen-specific CD4+ T cell responses of either Th1 or Th2 character for use in antitumour therapy or allograft facilitation, respectively. Similar obstacles exist in murine and human cultures: (1) during even brief periods of culture CD4+ T cells develop high 'background' reactivity to class II-positive antigen-presenting cells; (2) maintenance of antigen specificity as evidenced by cytokine secretion and short-term proliferation assays is insufficient to ensure bulk numerical expansion; (3) Th1-type CD4+ T cells often lose their potential for antigen-specific secretion of interleukin 2 on re-stimulation (though remain inducible by 12-O-tetradecanoylphorbol 13-acetate/ionomycin); (4) during prolonged culture selection pressure favours CD4+ subpopulations that recognize artifactual antigens such as culture medium protein...Continue Reading

Citations

Oct 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Liaomin PengPeter A Cohen
Dec 20, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·L PengP A Cohen
Nov 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·L PengP A Cohen

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