Propanil inhibits tumor necrosis factor-alpha production by reducing nuclear levels of the transcription factor nuclear factor-kappab in the macrophage cell line ic-21

Toxicology and Applied Pharmacology
L L FrostJ B Barnett

Abstract

Tumor necrosis factor-alpha (TNF-alpha) is an essential proinflammatory cytokine whose production is normally stimulated by bacterial cell wall components, such as lipopolysaccharide (LPS), during an infection. Macrophages stimulated with LPS in vitro produce several cytokines, including TNF-alpha. LPS-stimulated primary mouse macrophages produced less TNF-alpha protein and message after treatment with the herbicide propanil (Xie et al., Toxicol. Appl. Pharmacol. 145, 184-191, 1997). Nuclear factor-kappaB (NF-kappaB) tightly regulates TNF-alpha transcription. Therefore, as a step toward understanding the mechanism of the effect of propanil on TNF-alpha transcription, IC-21 cells were transfected with a TNF-alpha promoter-luciferase construct, and the effect of propanil on luciferase activity was measured. Cells transfected with promoter constructs containing a kappaB site showed decreased luciferase activity relative to controls after propanil treatment. These observations implicated NF-kappaB binding as an intracellular target of propanil. Further studies demonstrated a marked reduction in the nuclear levels of the stimulatory p65 subunit of NF-kappaB after propanil treatment, as measured by fluorescence confocal microscopy an...Continue Reading

References

Aug 12, 1994·Science·E Kopp, S Ghosh
Oct 7, 1998·Journal of Toxicology and Environmental Health. Part a·W ZhaoJ B Barnett
Jan 5, 2000·Cell·S Nakielny, G Dreyfuss

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Citations

Sep 30, 2008·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Keith D SalazarRosana Schafer
Jan 25, 2008·Environmental Toxicology·Gary O RankinJohn B Barnett
Mar 12, 2015·Toxicology Mechanisms and Methods·Aram MokarizadehMohammad Abdollahi
Mar 15, 2005·Journal of Toxicology and Environmental Health. Part a·Patricia de la RosaRosana Schafer
Dec 31, 2002·Biochemical Pharmacology·Sabrina DevosElisabeth L Hooghe-Peters
Nov 13, 2003·Journal of Toxicology and Environmental Health. Part a·Kathleen M BrundageJames E Mahaney
Dec 2, 2008·Human & Experimental Toxicology·E CorsiniC Colosio
Oct 8, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J B BoonyaratanakornkitM Hughes-Fulford
Oct 11, 2020·Fish & Shellfish Immunology·Magdalena MaciuszekMagdalena Chadzińska
Aug 8, 2021·Cells·Karolina NowakWioletta Ratajczak-Wrona

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