Proper regulation of cyclic AMP-dependent protein kinase is required for growth, conidiation, and appressorium function in the anthracnose fungus Colletotrichum lagenarium

Molecular Plant-microbe Interactions : MPMI
Yoshitaka TakanoT Okuno

Abstract

Colletotrichum lagenarium, the casual agent of anthracnose of cucumber, forms specialized infection structures, called appressoria, during infection. To evaluate the role of cAMP signaling in C. lagenarium, we isolated and functionally characterized the regulatory subunit gene of the cAMP-dependent protein kinase (PKA). The RPK1 gene encoding the PKA regulatory subunit was isolated from C. lagenarium by polymerase chain reaction-based screening. rpk1 mutants, generated by gene replacement, exhibited high PKA activity during vegetative growth, whereas the wild-type strain had basal level activity. The rpk1 mutants showed significant reduction in vegetative growth and conidiation. Furthermore, the rpk1 mutants were nonpathogenic on cucumber plants, whereas they formed lesions when inoculated through wounds. A suppressor mutant showing restored growth and conidiation was isolated from a rpk1 mutant culture. The rpkl-suppressor mutant did not show high PKA activity, unlike the parental rpk1 mutant, suggesting that high PKA activity inhibits normal growth and conidiation. The suppressor mutant, however, was nonpathogenic on cucumber and failed to form lesions, even when inoculated through wounds. The rpk1 and suppressor mutants form...Continue Reading

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Citations

Aug 4, 2010·Applied Microbiology and Biotechnology·Takumi TakedaRyohei Terauchi
Oct 12, 2012·World Journal of Microbiology & Biotechnology·Hsieh-Chin TsaiKuang-Ren Chung
Mar 7, 2003·Fungal Genetics and Biology : FG & B·Kim LangfelderAxel A Brakhage
Jan 26, 2011·Molecular Plant-microbe Interactions : MPMI·Hye-Seon KimSeogchan Kang
Oct 10, 2008·Molecular Plant-microbe Interactions : MPMI·Julia SchumacherBettina Tudzynski
May 15, 2003·Molecular Plant-microbe Interactions : MPMI·Gento TsujiYasuyuki Kubo
Dec 16, 2004·Molecular Plant-microbe Interactions : MPMI·Junko YamauchiTetsuro Okuno
Jun 10, 2008·Applied and Environmental Microbiology·Christina GrosseAxel A Brakhage
Sep 8, 2006·Applied and Environmental Microbiology·Makoto AsakuraYoshitaka Takano
Mar 26, 2003·Annual Review of Phytopathology·Nancy LeeJames W Kronstad
Nov 4, 2015·Plant Signaling & Behavior·Akifumi SugiyamaHiroshi Sano
Aug 8, 2015·Environmental Microbiology·Joon-Hee HanKyoung Su Kim
Jul 13, 2007·Fungal Genetics and Biology : FG & B·Carmit ZivOded Yarden
Mar 5, 2013·The Plant Journal : for Cell and Molecular Biology·Shailendra SharmaHiromasa Saitoh
May 3, 2016·Seminars in Cell & Developmental Biology·Kou Yanjun, Naweed I Naqvi
Jun 3, 2016·Journal of Basic Microbiology·Meenakshi SharmaSaurabh Kulshrestha
Nov 3, 2010·Molecular Plant-microbe Interactions : MPMI·Ayumu SakaguchiYasuyuki Kubo
Dec 7, 2017·Frontiers in Microbiology·Poonguzhali SelvarajNaweed I Naqvi
Sep 3, 2004·Molecular Microbiology·Kaihei KojimaTetsuro Okuno
Dec 26, 2018·Proceedings of the National Academy of Sciences of the United States of America·Hiroki IriedaYoshitaka Takano

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