Proper splicing contributes to visual function in the aging Drosophila eye

Aging Cell
R StegemanVikki M Weake

Abstract

Changes in splicing patterns are a characteristic of the aging transcriptome; however, it is unclear whether these age-related changes in splicing facilitate the progressive functional decline that defines aging. In Drosophila, visual behavior declines with age and correlates with altered gene expression in photoreceptors, including downregulation of genes encoding splicing factors. Here, we characterized the significance of these age-regulated splicing-associated genes in both splicing and visual function. To do this, we identified differential splicing events in either the entire eye or photoreceptors of young and old flies. Intriguingly, aging photoreceptors show differential splicing of a large number of visual function genes. In addition, as shown previously for aging photoreceptors, aging eyes showed increased accumulation of circular RNAs, which result from noncanonical splicing events. To test whether proper splicing was necessary for visual behavior, we knocked down age-regulated splicing factors in photoreceptors in young flies and examined phototaxis. Notably, many of the age-regulated splicing factors tested were necessary for proper visual behavior. In addition, knockdown of individual splicing factors resulted in ...Continue Reading

References

Oct 15, 1976·Developmental Biology·D F ReadyS Benzer
Nov 1, 1971·Kybernetik·N Franceschini, K Kirschfeld
Apr 19, 2003·Science·Annachiara De Sandre-GiovannoliNicolas Lévy
Jul 31, 2003·Molecular Cell·Melissa S Jurica, Melissa J Moore
Sep 2, 2004·Nature Reviews. Molecular Cell Biology·Chanseok Shin, James L Manley
Jun 16, 2005·Nature Reviews. Molecular Cell Biology·Arianne J MatlinChristopher W J Smith
Sep 28, 2006·Investigative Ophthalmology & Visual Science·Lori S SullivanStephen P Daiger
Jan 4, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Carolina Mendoza-TopazJimena Sierralta
Nov 4, 2008·Nature·Eric T WangChristopher B Burge
Mar 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Satoko YamaguchiMartin Heisenberg
Mar 26, 2010·Nature·Cynthia J Kenyon
Dec 24, 2010·Nature·Brenton R GraveleySusan E Celniker
Mar 3, 2012·G3 : Genes - Genomes - Genetics·T MukherjeeUtpal Banerjee
Apr 4, 2012·Frontiers in Physiology·Francisco Javier Blanco, Carmelo Bernabéu
Jan 24, 2013·Molecular Systems Biology·Pavel MazinPhilipp Khaitovich
May 25, 2013·Clinical Genetics·S P DaigerS J Bowne
Jun 12, 2013·Mechanisms of Ageing and Development·Alice C HollyLorna W Harries
Jan 24, 2014·Nature Reviews. Molecular Cell Biology·A Gregory Matera, Zefeng Wang
Feb 2, 2015·Current Opinion in Neurobiology·Roger C Hardie, Mikko Juusola
Aug 22, 2015·Genome Research·Angela N BrooksSteven E Brenner
Apr 27, 2016·Proceedings of the National Academy of Sciences of the United States of America·Qingqing WangDonald C Rio
Aug 31, 2016·RNA Biology·Jeremy E Wilusz
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Mary Anna CarboneTrudy F C Mackay
Dec 14, 2016·Scientific Reports·Hannah GrunerPedro Miura
Mar 28, 2017·Molecular Cell·Nagarjuna Reddy PamudurtiSebastian Kadener
Mar 28, 2017·Molecular Cell·Ivano LegniniIrene Bozzoni
May 26, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Eugenia C OlesnickyMeghan C Lybecker
Jul 8, 2017·Journal of Molecular Biology·R Stegeman, V M Weake
Jul 14, 2017·Aging Cell·Mathieu Deschênes, Benoit Chabot
Dec 8, 2017·Npj Aging and Mechanisms of Disease·Xinping ChenVikki M Weake

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Citations

Nov 18, 2018·Canadian Journal of Physiology and Pharmacology·Akash K GeorgeMahavir Singh
Dec 14, 2019·Human Genetics·Malini BhadraWilliam B Mair
Feb 11, 2021·Wiley Interdisciplinary Reviews. RNA·Brittany Lynn Angarola, Olga Anczuków
May 22, 2021·Trends in Genetics : TIG·Eunah KimSeung-Jae V Lee

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Datasets Mentioned

BETA
GSE83431
GSE106652

Methods Mentioned

BETA
JunctionSeq

Software Mentioned

DESeq2
JunctionSeq

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