PMID: 6112200Mar 1, 1981Paper

Properties and biosynthetic connection of the nucleotide pyrophosphatases of rat liver plasma membrane and endoplasmic reticulum

Hoppe-Seyler's Zeitschrift für physiologische Chemie
T T TranK Decker

Abstract

The detergent-solubilized nucleotide pyrophosphatases of the rat liver plasma membrane and endoplasmic reticulum fractions were purified by lectin affinity chromatography. They have the same molecular mass of 148 000 dalton; their catalytic properties are also very similar and correspond to those of the trypsin-solubilized activities from the same membrane preparations. Pulse-chase experiments on isolated perfused livers using [3H]leucine indicated different labelling kinetics of the proteins isolated from plasma membrane and endoplasmic reticulum. The plasma membrane enzyme became only slightly labelled in the presence of 100 microM vinblastine. The data support a precursor-product relationship of the nucleotide pyrophosphatases from endoplasmic reticulum and plasma membrane.

References

Sep 24, 1975·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·K Kovacs, E Horvath
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·C HomcyE Haber
Jan 1, 1971·Hoppe-Seyler's Zeitschrift für physiologische Chemie·W DomschkeK Decker
Jul 1, 1971·The Journal of Cell Biology·C de Duve

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Citations

Dec 15, 1987·European Journal of Biochemistry·D HäussingerK Decker
Nov 1, 1982·European Journal of Biochemistry·G Weckbecker, D O Keppler

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