Properties and molecular identity of NMDA receptors at synaptic and non-synaptic inputs in cerebellar molecular layer interneurons

Frontiers in Synaptic Neuroscience
Céline BidoretMariano Casado

Abstract

N-methyl-D-aspartate receptors (NMDARs) in cerebellar molecular layer interneurons (MLIs) are expressed and activated in unusual ways: at parallel fibre (PF) synapses they are only recruited by repetitive stimuli, suggesting an extrasynaptic location, whereas their activation by climbing fibre is purely mediated by spillover. NMDARs are thought to play an important role in plasticity at different levels of the cerebellar circuitry. Evaluation of the location, functional properties and physiological roles of NMDARs will be facilitated by knowledge of the NMDAR isoforms recruited. Here we show that MLI-NMDARs activated by both PF and climbing fibre inputs have similar kinetics and contain GluN2B but not GluN2A subunits. On the other hand, no evidence was found of functional NMDARs in the axons of MLIs. At the PF-Purkinje cell (PF-PC) synapse, the activation of GluN2A-containing NMDARs has been shown to be necessary for the induction of long-term depression (LTD). Our results therefore provide a clear distinction between the NMDARs located on MLIs and those involved in plasticity at PF-PC synapses.

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Citations

Jan 5, 2016·Trends in Neurosciences·Abhishek BanerjeeOle Paulsen
Nov 26, 2015·Neuroscience·Guy BouvierPierre Paoletti
Nov 2, 2018·The Journal of Physiology·Maya KonoMichisuke Yuzaki
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Nov 4, 2020·The Journal of Physiology·Hovy Ho-Wai WongP Jesper Sjöström
Feb 18, 2021·Scientific Reports·Martina Francesca RizzaEgidio D'Angelo
Sep 12, 2021·Proceedings of the National Academy of Sciences of the United States of America·Martijn SchonewilleGuy Bouvier

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Methods Mentioned

BETA
electron microscopy

Software Mentioned

JaCoP
pCLAMP8
R ( R Foundation for Statistical Computing
GNU
ImageJ

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