Properties and origin of spikelets in thalamocortical neurones in vitro

Neuroscience
Stuart W HughesVincenzo Crunelli

Abstract

Spikelets, or fast prepotentials as they are frequently referred to, are a common feature of the electrophysiology of central neurones and are invariably correlated with the presence of electrotonic coupling via gap junctions. Here we report that in the presence of the metabotropic glutamate receptor agonists, trans-ACPD or DHPG, thalamocortical neurones of the cat dorsal lateral geniculate nucleus maintained in vitro exhibit stereotypical spikelets that possess similar properties to those described in other brain areas. These spikelets were routinely observed in the presence of antagonists of fast chemical synaptic transmission, were resistant to the application of a variety of voltage-dependent Ca(2+) channel blockers but were abolished by tetrodotoxin. In addition, spikelets were reversibly blocked by the putative gap junction blocker carbenoxolone and were nearly always accompanied by dye-coupling. These results indicate that thalamocortical neurones may be electrotonically coupled via gap junctions with spikelets representing attenuated action potentials from adjoining cells. We suggest that the presence of electrotonic communication between thalamocortical neurones would have major implications for the understanding of bo...Continue Reading

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Citations

Sep 6, 2011·Pflügers Archiv : European journal of physiology·Vincenzo CrunelliStuart W Hughes
Jul 17, 2013·Clinical Neurophysiology : Official Journal of the International Federation of Clinical Neurophysiology·Kazuko HayashiTeiji Sawa
Dec 8, 2009·Nature Neuroscience·Vincenzo Crunelli, Stuart W Hughes
Mar 11, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Vincenzo CrunelliStephen R Williams
Feb 13, 2009·PloS One·Magor L LörinczStuart W Hughes
Sep 25, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hong-Wei DongMatthew Ennis
Dec 7, 2007·Clinical Neurophysiology : Official Journal of the International Federation of Clinical Neurophysiology·Angelo InsolaMassimiliano Valeriani
Oct 21, 2003·The Journal of Comparative Neurology·Xiao-Bo Liu, Edward G Jones
Sep 9, 2006·The Journal of Physiology·Marie-Claude Perreault, Morten Raastad
May 12, 2010·The Journal of Physiology·Seung-Chan LeeBarry W Connors
Sep 27, 2006·International Journal of Psychophysiology : Official Journal of the International Organization of Psychophysiology·Stuart W Hughes, Vincenzo Crunelli
Jan 20, 2006·The European Journal of Neuroscience·Eliane ProulxJose L Perez Velazquez
Sep 19, 2014·Journal of Autism and Developmental Disorders·J Christopher EdgarTimothy P L Roberts
May 3, 2016·Neuron·Miklos SzoboszlayZoltan Nusser
Dec 11, 2007·Journal of Neuroscience Methods·Stuart W HughesVincenzo Crunelli
Aug 3, 2005·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Stuart W Hughes, Vincenzo Crunelli
Aug 6, 2004·Journal of Neurophysiology·Ritsuko HanajimaJonathan O Dostrovsky
Oct 16, 2004·Journal of Neurophysiology·Giulietta Pinato, Jens Midtgaard
Apr 13, 2019·Human Brain Mapping·J Christopher EdgarTimothy P L Roberts
Aug 23, 2019·Reviews in the Neurosciences·Martina MichalikovaRichard Kempter
Feb 26, 2021·Journal of Autism and Developmental Disorders·Heather L GreenJ Christopher Edgar

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