PMID: 2125266Dec 12, 1990Paper

Properties and partial characterization of the heat-shock factor from Tetrahymena pyriformis

European Journal of Biochemistry
M do C AvidesC Rodrigues-Pousada

Abstract

A heat-shock-factor-binding activity was identified in Tetrahymena pyriformis whole-cell extracts and was further purified by sequential heparin-agarose and sequence-specific oligonucleotide affinity chromatography. Tetrahymena heat-shock factor (HSF) was able to bind to the heat-shock elements (HSE) both before and after thermal stress, although heat shock altered both the HSE-binding affinity and the protein.DNA-complex mobility on polyacrylamide gels. The mobility difference was significantly reduced by treatment of the proteins with phosphatase. The HSE-binding proteins, isolated by oligonucleotide-affinity chromatography, migrated on SDS/polyacrylamide gels as a closely spaced doublet to about 70 kDa. Polypeptides with similar molecular mass were recovered from preparative band-shift gels indicating that both are components of the protein.DNA complex.

References

Jun 18, 1985·European Journal of Biochemistry·L Galego, C Rodrigues-Pousada
Jun 1, 1988·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·R M Tanguay
Nov 1, 1988·Molecular and Cellular Biology·B K Jakobsen, H R Pelham
Feb 1, 1988·European Journal of Biochemistry·M D AmaralC Rodrigues-Pousada
Sep 1, 1988·Molecular and Cellular Biology·J AminR Voellmy
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J T Kadonaga, R Tjian
Apr 1, 1987·Molecular and Cellular Biology·R E KingstonZ Larin
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R Voellmy, D Rungger

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Citations

Jul 4, 2012·Acta Microbiologica Et Immunologica Hungarica·Eszter LajkóGyörgy Csaba
Oct 1, 1995·Protein Science : a Publication of the Protein Society·O H KappS N Vinogradov
Jul 1, 1993·Molecular & General Genetics : MGG·E TreuterK D Scharf
Aug 19, 1993·Biochimica Et Biophysica Acta·M D AmaralC Rodrigues-Pousada
Jun 1, 1994·Microbiological Reviews·D M Prescott

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