Properties and regulation of glutamine transporter SN1 by protein kinases SGK and PKB

Biochemical and Biophysical Research Communications
Christoph BoehmerFlorian Lang

Abstract

The amino acid transporter SN1 with substrate specificity identical to the amino acid transport system N is expressed mainly in astrocytes and hepatocytes where it accomplishes Na(+)-coupled glutamine uptake and efflux. To characterize properties and regulation of SN1, substrate-induced currents and/or radioactive glutamine uptake were determined in Xenopus oocytes injected with cRNA encoding SN1, the ubiquitin ligase Nedd4-2, and/or the constitutively active serum and glucocorticoid inducible kinase S422DSGK1, its isoform SGK3, and the constitutively active protein kinase B T308D,S473DPKB. The substrate-induced currents were enhanced by increasing glutamine and/or Na(+) concentrations, hyperpolarization, and alkalinization (pH 8.0). They were inhibited by acidification (pH 6.0). Coexpression of Nedd4-2 downregulated SN1-mediated transport, an effect reversed by coexpression of S422DSGK1, SGK3, and T308D,S473DPKB. It is concluded that SN1 is a target for the ubiquitin ligase Nedd4-2, which is inactivated by the serum and glucocorticoid inducible kinase SGK1, its isoform SGK3, and protein kinase B.

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Citations

Feb 18, 2006·Pflügers Archiv : European journal of physiology·Nathalie Strutz-SeebohmFlorian Lang
Jun 23, 2005·Pflügers Archiv : European journal of physiology·Ciprian SanduFlorian Lang
Oct 20, 2006·Pflügers Archiv : European journal of physiology·Rexhep RexhepajFlorian Lang
Oct 25, 2011·Neuron Glia Biology·Jan AlbrechtMichael Aschner
Feb 23, 2010·The Journal of Biological Chemistry·Jian WangWannian Yang
Aug 6, 2010·American Journal of Physiology. Cell Physiology·Irina M ZemtsovaFlorian Lang
Nov 6, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Perikles SimonFlorian Lang
Nov 6, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Nathalie Strutz-SeebohmFlorian Lang
Jan 23, 2008·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Matthias SchwabFlorian Lang
Jan 12, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Alexei DiakovChristoph Korbmacher
Apr 19, 2013·BMC Pharmacology & Toxicology·Marta Sidoryk-Wegrzynowicz, Michael Aschner
May 27, 2014·Trends in Endocrinology and Metabolism : TEM·Kenta MasuiPaul S Mischel
Jan 23, 2009·American Journal of Physiology. Renal Physiology·Teresa F AckermannFlorian Lang
Jun 2, 2011·Journal of Pharmaceutical Sciences·Takeo Nakanishi, Ikumi Tamai
Mar 27, 2007·Biochemical and Biophysical Research Communications·Sankarganesh JeyarajMonica Palmada
May 22, 2009·Journal of Neurochemistry·Marta Sidoryk-WegrzynowiczMichael Aschner
Jun 16, 2007·Biochemical and Biophysical Research Communications·Manzar ShojaiefardFlorian Lang
Apr 23, 2005·Biochemical and Biophysical Research Communications·Monica PalmadaFlorian Lang
Jan 31, 2013·Journal of Internal Medicine·M Sidoryk-Wegrzynowicz, M Aschner
Dec 2, 2004·Biochimica Et Biophysica Acta·S DupréR Haguenauer-Tsapis
Jan 31, 2006·Diabetes·Monica PalmadaFlorian Lang
Feb 10, 2006·Biochemical and Biophysical Research Communications·Manzar ShojaiefardFlorian Lang
Oct 27, 2004·Biochemical and Biophysical Research Communications·Christoph BöhmerFlorian Lang
Jul 23, 2005·Biochemical and Biophysical Research Communications·Manzar ShojaiefardFlorian Lang
Mar 2, 2005·Biochemical and Biophysical Research Communications·Christoph BoehmerFlorian Lang
Mar 22, 2018·International Journal of Molecular Sciences·Wuxing DongBrian Billups
Jun 22, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Cai TangFlorian Lang
Dec 14, 2012·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Evgenii BogatikovFlorian Lang
Jul 28, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Ge-Xin WangIlia A Yamboliev
Oct 19, 2004·The Journal of Biological Chemistry·Deanne H HryciwPhilip Poronnik
Apr 19, 2006·Journal of Cellular Biochemistry·Maude Tessier, James R Woodgett
Jun 14, 2005·American Journal of Physiology. Renal Physiology·Maria Francisca Arteaga, Cecilia M Canessa
Jan 18, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Florian GrahammerFlorian Lang
Nov 23, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Yuxi FengFlorian Lang
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