PMID: 6982243Jan 1, 1982Paper

Properties and specificity of binding sites for the immunomodulator bestatin on the surface of mammalian cells

International Journal of Immunopharmacology
W E MüllerH Umezawa

Abstract

The binding of the immunomodulator bestatin, an inhibitor of cell surface bound leucine aminopeptidase and aminopeptidase B, to mammalian cells of varying origin has been studied. The specific binding of [3H] bestatin was a rapid and saturable process exhibiting one affinity, characterized by an association constant of 0.8 x 10(5) M-1, as determined in the L5178y mouse lymphoma system. Optimal binding was observed at 37 degrees C. L-leucine and L-leucine-beta-naphthylamide prevented the binding, suggesting that the complex was formed between leucine aminopeptidase and bestatin. The protein nature of the bestatin-"receptor" was suggested by its susceptibility to trypsin. Under the conditions used here intracellular translocation of bestatin appeared to be negligible. A maximum of about 2.2 x 10(6) bestatin molecules could bind to L5178y mouse lymphoma cells. Under identical conditions by far the highest amount of bestatin was bound to macrophages from mice. Lower levels were measured with T-lymphocytes; very low binding capacity was observed with B-lymphocytes. Experiments with synchronized L5178y cells revealed a cell cycle dependent change of binding capacity for bestatin; the highest level was observed during the transition f...Continue Reading

References

Oct 15, 1979·Biochemical Pharmacology·W E MüllerH Umezawa
Jan 1, 1976·The Journal of Antibiotics·H UmezawaT Takeuchi
May 1, 1976·The Journal of Antibiotics·H SudaH Umezawa
Nov 8, 1976·Biochimica Et Biophysica Acta·T AoyagiJ Suzuki
Nov 1, 1976·Archives of Biochemistry and Biophysics·H SudaH Umezawa
Mar 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·I Yahara, G M Edelman
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg
Jul 1, 1973·Experimental Cell Research·W E Müller, R K Zahn
Jun 1, 1980·The Journal of Antibiotics·M IshizukaH Umezawa
Jun 1, 1980·The Journal of Antibiotics·M IshizukaH Umezawa

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Citations

Jan 1, 1992·Biotherapy·K Ota, Y Uzuka
Feb 1, 1986·Journal of the Neurological Sciences·S Tsuji, H Matsushita
May 1, 1992·Immunopharmacology·K InoY Tomoda
Jan 1, 1990·International Journal of Immunopharmacology·T YamashitaM Enomoto
Jan 1, 1991·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·K Ota
Jan 1, 1991·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·J E TalmadgeF Abe
Jan 1, 1995·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·T FujisakiY Niho
Apr 17, 2003·Journal of the National Cancer Institute·Yukito IchinoseUNKNOWN NK421 Lung Cancer Surgery Group
Sep 1, 1990·The Japanese Journal of Psychiatry and Neurology·M KishiM Kinoshita
Feb 3, 2007·International Journal of Cancer. Journal International Du Cancer·Mamoru YamashitaFumitaka Kikkawa
Sep 1, 1984·Scandinavian Journal of Immunology·B E DunlapD H Rich
Jan 1, 1985·Biotechnology & Genetic Engineering Reviews·H Umezawa
May 24, 2006·Bioscience, Biotechnology, and Biochemistry·Koshiro Migita, Toshihide Nishimura
Dec 4, 2012·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Federico BecerraAldo Iván Vassallo
May 29, 2000·American Journal of Hypertension·V PetrovP Lijnen
Oct 1, 1984·Hoppe-Seyler's Zeitschrift für physiologische Chemie·K H Röhm

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