Properties and submitochondrial localization of pig and rat renal phosphate-activated glutaminase

American Journal of Physiology. Cell Physiology
B RobergE Kvamme

Abstract

Two pools of phosphate-activated glutaminase (PAG) were separated from pig and rat renal mitochondria. The partition of enzyme activity corresponded with that of the immunoreactivity and also with the postembedding immunogold labeling of PAG, which was associated partly with the inner membrane and partly with the matrix. The outer membrane was not labeled. PAG in intact mitochondria showed enzymatic characteristics that were similar to that of the membrane fraction and also mimicked that of the polymerized form of purified pig renal PAG. PAG in the soluble fraction showed properties similar to that of the monomeric form of purified enzyme. It is indicated that the pool of PAG localized inside the inner mitochondrial membrane is dormant due to the presence of high concentrations of the inhibitor glutamate. Thus the enzymatically active PAG is assumed to be localized on the outer face of the inner mitochondrial membrane. The activity of this pool of PAG appears to be regulated by compounds in the cytosol, of which glutamate may be most important.

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Citations

Sep 10, 2002·American Journal of Physiology. Endocrinology and Metabolism·Greg CoatesTomas Welbourne
Oct 29, 2017·Neurochemical Research·Hong Q SmithThomas James Smith
May 16, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Tomas WelbourneHarold Battarbee
Sep 1, 2004·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Lynn Taylor, Norman P Curthoys
Feb 16, 2013·Neurochemical Research·Jason R TrebergDirk Weihrauch
Apr 12, 2012·The Journal of Biological Chemistry·Kayoko KitaTakafumi Ochi
Aug 30, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mahmood Amiry-MoghaddamOle P Ottersen
Aug 2, 2001·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·T WelbourneI Nissim
Mar 27, 2001·Biochimica Et Biophysica Acta·L TaylorN P Curthoys
Dec 18, 2001·Journal of Neuroscience Research·E KvammeB Roberg

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