Properties of a gonococcal inhibitor produced by Escherichia coli

Journal of General Microbiology
D M Simpson, C P Davis

Abstract

Strains of Escherichia coli can inhibit the in vitro growth of Neisseria gonorrhoeae. One E. coli strain released a potent agar-diffusible gonococcal growth inhibitor which was extracted and assayed in an agar well assay system. The culture conditions necessary to produce the inhibitor were determined. The inhibitor was bacteriostatic, in most cases, for N. gonorrhoeae. Based on ultrafiltration and column chromatography, the inhibitor appeared to have a molecular weight in the range of 1200 to 2000. Evidence that the molecule contained charged sites was obtained by membrane binding and column chromatography. The inhibitor was stable to extremes of heat, cold and pH. It was not volatile or susceptible to proteolytic enzymes, lysozyme, lipase, DNAase, RNAase or certain chelating agents. Its activity was completely blocked by ferric ammonium citrate. This inhibitor is dissimilar to previously reported gonococcal inhibitors of bacterial origin.

Citations

Dec 1, 1983·Journal of Adolescent Health Care : Official Publication of the Society for Adolescent Medicine·E A Schaff
Nov 1, 1990·International Journal of STD & AIDS·J Hare

Related Concepts

Alkalescens-Dispar Group
Ferric Compounds
Growth Inhibitors
Hydrogen-Ion Concentration
Neisseria gonorrhoeae

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