Properties of a hemolysin produced by group B streptococci.

Infection and Immunity
B A Marchlewicz, J L Duncan

Abstract

A hemolysin that appears to be responsible for the zones of beta-hemolysis surrounding colonies of group B streptococci (Streptococcus agalactiae) on blood agar plates has been isolated and partially purified. No soluble hemolysin was detectable in the supernatants of streptococcal cultures grown in several types of media. However, hemolytic activity was detected when streptococci were incubated with erythrocytes, and soluble hemolysin was isolated when bacterial suspensions were incubated in the presence of a variety of agents, including calf serum, albumin, Tween 80, and starch. Glucose and other fermentable carbohydrates stimulated hemolysin production, and metabolic inhibitors greatly reduced the titer of hemolysin that could be recovered, suggesting that cellular metabolism is necessary for hemolysin production or release. The soluble hemolysin was concentrated by ammonium sulfate precipitation and partially purified by gel filtration. Agents known to inhibit other streptococcal hemolysins, including phospholipids, trypan blue, proteases, and cholesterol, were tested for their effect on the group B hemolysin. Only the phospholipids inhibited hemolysin activity. The group B streptococcal hemolysin appears to be similar to, ...Continue Reading

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Citations

Feb 8, 2003·Journal of Clinical Microbiology·Norio OkazakiRobert A Whiley
Aug 4, 2006·Journal of Bacteriology·Birgit GottschalkBarbara Spellerberg
May 1, 1994·Journal of Clinical Microbiology·I FederJ Staats
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Jan 1, 1994·Infection and Immunity·L V Stamm, E A Parrish
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Feb 1, 1992·FEMS Microbiology Immunology·B L WetherallA M Johnson
Jan 1, 1990·Journal of Basic Microbiology·B B Griffiths
Jul 5, 2008·Microbial Pathogenesis·Amber KaplanOzlem Equils

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