PMID: 8603081Mar 13, 1996Paper

Properties of a Na+/galactose (glucose) symport system in Vibrio parahaemolyticus

Biochimica Et Biophysica Acta
R I SarkerT Tsuchiya

Abstract

We have investigated galactose transport in a mutant strain of Vibrio parahaemolyticus that lacks a glucose-PTS (phosphoenolpyruvate:carbohydrate phosphotransferase system) and a trehalose-PTS. Cells of the V. parahaemolyticus actively transported D-galactose and Na+ greatly stimulated the transport. Maximum stimulation of D-galactose transport activity was observed at 10mM NaCl, and Na+ could be replaced with Li+. Addition of galactose to the cell suspension under anaerobic conditions elicited Na+ uptake. Therefore, we conclude that this organism accomplishes galactose transport by a Na+/solute symport mechanism. Judging from inhibition results, D-galactose, D-glucose and to a lesser extent alpha-D-fucose are substrates of this transport system. The Na+/galactose symport system exhibited a high affinity for D-galactose (Km: 40 microM) and showed a relatively lower affinity for D-glucose (Km: 420 microM), but the maximum velocities for galactose and glucose transport were almost same (about 52 nmol/min per mg protein). The Na+/D-galactose symport system was induced by either D-galactose or alpha-D-fucose, and repressed by D-glucose.

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Citations

Nov 14, 2000·Journal of Bacteriology·Y MoritaT Tsuchiya
Dec 23, 2008·Biochimica Et Biophysica Acta·Teruo Kuroda, Tomofusa Tsuchiya
Jul 24, 2008·FEMS Microbiology Reviews·Knut JahreisFritz Titgemeyer
Mar 15, 2001·Biochimica Et Biophysica Acta·H Jung

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