Abstract
A highly penicillin-resistant strain of Bacteroides fragilis, strain GN11499, was found among 80 clinical isolates of the B.fragilis group and appears to produce a new type of penicillinase. Penicillinase activity was detected in crude extracts and had a specific activity of 0.25 U/mg of protein. About 20% of the enzyme was released into the surrounding medium during growth. The enzyme hydrolyzed ampicillin and cloxacillin more rapidly than it did penicillin G, carbenicillin, and cephaloridine. Relative rates in a crude extract with penicillin G as 100 were ampicillin, 357; carbenicillin, 57; cloxacillin, 271; and cephaloridine, 71. Enzyme activity was inhibited by clavulanic acid, CP-45, 899, N-formimidoyl thienamycin, cefoxitin, moxalactam, and p-chloromercuribenzoate. The enzyme had a molecular weight of approximately 41,500 and an isoelectric point of 6.9. Penicillinase production and tetracycline resistance were transferred from B.fragilis GN11499 to two susceptible strains of B.fragilis and Bacteroides vulgatus by filter mating.
References
May 1, 1976·Antimicrobial Agents and Chemotherapy·B OlssonT Wadström
Mar 1, 1978·Antimicrobial Agents and Chemotherapy·M L Britz, R G Wilkinson
Jan 1, 1975·Scandinavian Journal of Infectious Diseases·K DornbuschB Olsson
Mar 1, 1978·Antimicrobial Agents and Chemotherapy·F P TallyS L Gorbach
Sep 1, 1978·Antimicrobial Agents and Chemotherapy·A R EnglishW E Barth
Nov 1, 1978·Antimicrobial Agents and Chemotherapy·H C Neu, K P Fu
Aug 1, 1979·Antimicrobial Agents and Chemotherapy·H C NeuP Aswapokee
Dec 1, 1979·Antimicrobial Agents and Chemotherapy·N MatsubaraS Mitsuhashi
Apr 12, 1979·Nature·G PriviteraF Fayolle
Apr 1, 1979·Plasmid·R A WelchF L Macrina
Oct 1, 1976·Antimicrobial Agents and Chemotherapy·V L Sutter, S M Finegold
Feb 1, 1976·Journal of Bacteriology·M Matthew, R W Hedges
Jun 1, 1974·The Biochemical Journal·S G Waley
Sep 1, 1974·Journal of General Microbiology·C E NordT Wadström
May 1, 1973·Journal of Medical Microbiology·J D Anderson, R B Sykes
Jan 1, 1973·Advances in Microbial Physiology·M H Richmond, R B Sykes
Aug 1, 1974·Journal of Bacteriology·J W Dale, J T Smith
Mar 1, 1973·Antimicrobial Agents and Chemotherapy·V E Del Bene, W E Farrar
Jul 1, 1969·Journal of Biochemistry·S YamagishiS Mitsuhashi
Jul 1, 1970·The Biochemical Journal·S Kuwabara
Jun 11, 1971·Annals of the New York Academy of Sciences·R P Novick, D Bouanchaud
Oct 1, 1968·Journal of Bacteriology·G PinkusA I Braude
Apr 1, 1980·Antimicrobial Agents and Chemotherapy·K SatoS Mitsuhashi
Nov 1, 1980·Antimicrobial Agents and Chemotherapy·M TodaS Mitsuhashi
Dec 24, 1955·British Medical Journal·L P GARROD
May 1, 1962·The Biochemical Journal·R P NOVICK
Citations
Jun 1, 1994·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·P MastrantonioA Sebastianelli
Apr 1, 1994·Diagnostic Microbiology and Infectious Disease·K E AldridgeD D Schiro
Apr 1, 1983·Antimicrobial Agents and Chemotherapy·F P TallyC L Pierson
Dec 1, 1983·Antimicrobial Agents and Chemotherapy·A YotsujiS Mitsuhashi
Dec 1, 1983·Antimicrobial Agents and Chemotherapy·G J CuchuralJ W Mayhew
Jan 1, 1984·Antimicrobial Agents and Chemotherapy·T KesadoK Ueno
Jun 1, 1985·Antimicrobial Agents and Chemotherapy·K TunérC E Nord
May 1, 1986·Antimicrobial Agents and Chemotherapy·G J CuchuralM H Malamy
Nov 1, 1986·Antimicrobial Agents and Chemotherapy·G J CuchuralF P Tally
Apr 1, 1987·Antimicrobial Agents and Chemotherapy·A E De Almeida, M De Uzeda
Mar 1, 1989·Antimicrobial Agents and Chemotherapy·K Bush
Aug 1, 1989·Antimicrobial Agents and Chemotherapy·T KesadoC E Nord
Nov 1, 1990·Antimicrobial Agents and Chemotherapy·P C AppelbaumL Gutmann
Jan 1, 1992·Antimicrobial Agents and Chemotherapy·K LeeY Chong
Apr 1, 1994·Antimicrobial Agents and Chemotherapy·K E AldridgeD D Schiro
Jun 1, 1995·Antimicrobial Agents and Chemotherapy·K BushA A Medeiros
Jan 1, 1984·Scandinavian Journal of Infectious Diseases. Supplementum
Feb 1, 1996·Journal of Chemotherapy·M Hedberg, C E Nord
Nov 27, 2002·Journal of Neurosurgery·Michael J LinkJohn M Tew
Jul 1, 1986·Drug Intelligence & Clinical Pharmacy·G J Cuchural, F P Tally
May 1, 1993·Journal of Bacteriology·C J Smith, A C Parker
Oct 1, 1991·Clinical Microbiology Reviews·H M Wexler
Mar 1, 1986·Journal of Clinical Microbiology·J H JorgensenA W Howell
Apr 1, 1996·Antimicrobial Agents and Chemotherapy·P MastrantonioP Spigaglia
Mar 1, 1997·Antimicrobial Agents and Chemotherapy·K E Aldridge, D S Ashcraft