PMID: 9557940Apr 29, 1998Paper

Properties of a polarized primary culture from rat renal inner medullary collecting duct (IMCD) cells

In Vitro Cellular & Developmental Biology. Animal
B RuhfusR K Kinne

Abstract

A primary culture from rat renal IMCD cells was established to investigate the permeability characteristics of the luminal and contraluminal plasma membranes of the papillary collecting duct in vitro. Freshly isolated IMCD cells were grown on filters in a special "epithelial cell" medium. Confluency was proved with an epithelial volt/ohm meter. After 7 d of culture the transepithelial resistance reached more than 1000 omega x cm2. A polarization of the cells with regard to a basolateral localization of a lactate efflux system, and an L-alanine transport system was achieved. The hypotonicity-activated release systems for the organic osmolytes sorbitol and betaine were also located basolaterally, whereas taurine, glycerophosphorylcholine, and myo-inositol left the cells at both cell poles but with different capacity. Morphological observations revealed also that the monolayer was well differentiated. Thus, a model of a renal collecting duct epithelium was established which can be used to analyze polarized and differentiated transport processes across the epithelial cells and their plasma membranes.

References

Jul 31, 1990·Biochemical and Biophysical Research Communications·C BevanR K Kinne
Nov 1, 1989·The American Journal of Physiology·N A SchroedlC R Hartzell
Oct 1, 1989·The American Journal of Physiology·T Nakanishi, M B Burg
Oct 1, 1987·Analytical Biochemistry·H K KleinmanG C Sephel
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr
Mar 1, 1982·The American Journal of Physiology·M BurgJ Handler
Jan 1, 1980·The American Journal of Physiology·L J MandelM M Grafton
Sep 1, 1993·Kidney International·J M GrunewaldR K Kinne
Jan 1, 1993·The Biochemical Journal·S O RosenbergV L Schuster
May 1, 1993·The American Journal of Physiology·S M BagnascoJ S Handler

❮ Previous
Next ❯

Citations

Dec 12, 2001·Kidney International·R W GrunewaldJ B Schuettert
Jul 22, 2014·Cytoskeleton·Lina C EspinhaChristopher R Jacobs
Sep 6, 2012·Biophysical Journal·Y-N YoungC R Jacobs
Mar 29, 2012·Computer Methods in Biomechanics and Biomedical Engineering·Matthew E DownsChristopher R Jacobs
Sep 21, 2010·Regulatory Peptides·Anthony L AlbistonSiew Yeen Chai
May 1, 1999·The Journal of Experimental Zoology·R W Grunewald, R K Kinne
Mar 30, 2001·The Journal of Biological Chemistry·Y V MukhinM N Garnovskaya
Sep 11, 2019·International Journal of Molecular Sciences·Annalisa ZiemensNina Himmerkus

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Computer Methods in Biomechanics and Biomedical Engineering
Matthew E DownsC R Jacobs
Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics
Y-N Young
Journal of Biomechanical Engineering
Kristopher R SchumacherAlexander A Spector
Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics
Tobias MunkErwin Frey
© 2022 Meta ULC. All rights reserved