PMID: 2509635Dec 1, 1989Paper

Properties of a protein kinase C activity in synaptic plasma membrane and postsynaptic density fractions isolated from canine cerebral cortex

Journal of Neurochemistry
T Suzuki, P Siekevitz

Abstract

Protein kinase C (PKC) activity (phosphorylation increased by addition of Ca2+/phosphatidylserine or Ca2+/phosphatidylserine/phorbol ester) was found in both a synaptic plasma membrane (SPM) and a postsynaptic density (PSD) fraction. The SPM fraction had as endogenous substrates 87K-, 60K-, 50K-, and 20K-Mr proteins, whereas the PSD fraction had only the 20K-Mr protein. The PKC activity was also detected using histone III-S as a substrate, in SPM but much less in PSD. Phosphorylations of histone and the endogenous substrates of PKC, assayed in the absence of Ca2+, were enhanced in the SPM prepared after treatment of brain homogenate with phorbol 12-myristate 13-acetate (TPA), but very little enhancement was found in PSD after such treatment. The SPM PKC activity (both for endogenous substrate proteins and for histone), which was enhanced by TPA treatment of brain homogenate, was inhibited by calcium (IC50, 3 x 10(-7) M). The phosphorylations of the 20K-Mr protein in PSD, and in SPM prepared with and without TPA treatment, were all inhibited by H-7. The 20K-Mr protein in the PSD fraction is also phosphorylated by a PSD Ca2+/calmodulin-dependent protein kinase II. The evidence indicates that both SPM and PSD fractions contain a P...Continue Reading

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·F E BloomP Greengard
May 14, 1986·Biochemical and Biophysical Research Communications·J EichbergW H Gispen
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P Siekevitz
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·E MelloniB L Horecker
Dec 31, 1985·Biochemical and Biophysical Research Communications·M R Costa-CasnellieM A Lichtman
Jan 29, 1986·Biochemical and Biophysical Research Communications·J NishihiraJ T O'Flaherty
Jul 18, 1986·Science·Y Nishizuka
Jul 23, 1986·Brain Research·A RouttenbergF S Sheu
Feb 1, 1987·Journal of Neurochemistry·R A NicholsP Greengard
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J M BarabanB E Alger
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P R GirardJ F Kuo
Jun 14, 1985·Biochemical and Biophysical Research Communications·J R McDonald, M P Walsh
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B BankD L Alkon
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D Mochly-RosenD E Koshland
Jan 1, 1987·Journal of Cellular Physiology. Supplement·P J Parker, A Ullrich
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K A AlbertP Greengard
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K P HuangF L Huang
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S Jaken, S C Kiley
May 1, 1987·Journal of Neurochemistry·L I BenowitzS P Finklestein
Jul 30, 1986·Brain Research·T Saitoh, K R Dobkins
Oct 30, 1986·Biochemical and Biophysical Research Communications·M WolfN Sahyoun
Sep 30, 1985·Biochemical and Biophysical Research Communications·K MizutaH Yamamura
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K A AlbertP Greengard

❮ Previous
Next ❯

Citations

Jun 1, 1994·The International Journal of Biochemistry·T Suzuki
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K Sunayashiki-KusuzakiD L Alkon
Aug 1, 1990·FEBS Letters·H Zimmermann
Aug 1, 1991·Journal of Neurochemistry·N V HayesA J Baines

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.