Properties of a transplasma membrane electron transport system in HeLa cells

Journal of Bioenergetics and Biomembranes
I L SunH Löw

Abstract

A transmembrane electron transport system has been studied in HeLa cells using an external impermeable oxidant, ferricyanide. Reduction of ferricyanide by HeLa cells shows biphasic kinetics with a rate up to 500 nmoles/min/g w.w. (wet weight) for the fast phase and half of this rate for the slow phase. The apparent Km is 0.125 mM for the fast rate and 0.24 mM for the slow rate. The rate of reduction is proportional to cell concentration. Inhibition of the rate by glycolysis inhibitors indicates the reduction is dependent on glycolysis, which contributes the cytoplasmic electron donor NADH. Ferricyanide reduction is shown to take place on the outside of cells for it is affected by external pH and agents which react with the external surface. Ferricyanide reduction is accompanied by proton release from the cells. For each mole of ferricyanide reduced, 2.3 moles of protons are released. It is, therefore, concluded that a transmembrane redox system in HeLa cells is coupled to proton gradient generation across the membrane. We propose that this redox system may be an energy source for control of membrane function in HeLa cells. The promotion of cell growth by ferricyanide (0.33-0.1 mM), which can partially replace serum as a growth ...Continue Reading

References

Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S E Hutchings, G H Sato
Jan 1, 1979·Sub-cellular Biochemistry·F L CraneH Löw
May 28, 1982·Science·D F GersonW Eufe
Oct 14, 1983·Biochemical and Biophysical Research Communications·I L SunC Grebing
Oct 20, 1982·Biochimica Et Biophysica Acta·H Goldenberg
Dec 1, 1982·Journal of Bioenergetics and Biomembranes·F L CraneH Löw
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·Z A TokesA Rembaum
Apr 15, 1983·Biochemical and Biophysical Research Communications·K A Ellem, G F Kay
Jun 1, 1982·Journal of Bioenergetics and Biomembranes·F L CraneH Löw
Jun 15, 1982·The Biochemical Journal·M G ClarkF L Crane
Dec 1, 1969·The Journal of Membrane Biology·R K Mishra, H Passow

❮ Previous
Next ❯

Citations

Dec 17, 2002·Arthritis and Rheumatism·Robert B Lee, Jill P G Urban
May 18, 2000·Biochemical and Biophysical Research Communications·P L LeeE Beutler
Dec 1, 1986·Journal of Bioenergetics and Biomembranes·I L SunH Löw
Jun 1, 1991·Journal of Bioenergetics and Biomembranes·P C Misra
Oct 1, 1991·Journal of Bioenergetics and Biomembranes·F L CraneH Löw
Dec 1, 1991·Journal of Bioenergetics and Biomembranes·H LöwI L Sun
Mar 1, 1996·Biulleten' eksperimental'noĭ biologii i meditsiny·A G GlobaA A Karelin
Jul 10, 1995·Archives of Biochemistry and Biophysics·J D Stahl, S D Aust
Jan 4, 1994·Biochimica Et Biophysica Acta·R Zurbriggen, J L Dreyer
Aug 14, 1987·Biochemical and Biophysical Research Communications·I L SunH Löw
May 29, 1987·Biochemical and Biophysical Research Communications·I L SunH Löw
Mar 31, 1994·Biochimica Et Biophysica Acta·I L SunH Löw
Mar 4, 1991·Biochimica Et Biophysica Acta·F J AlcainP Navas
Apr 9, 1991·Biochimica Et Biophysica Acta·A BércziW P Faulk
Aug 1, 1985·Biochimica Et Biophysica Acta·F L CraneH Löw
Sep 30, 1986·Biochemical and Biophysical Research Communications·H LöwD J Morre
Jan 22, 1998·Free Radical Biology & Medicine·N KaulH J Forman
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·I L SunH Löw
Mar 7, 2001·Antioxidants & Redox Signaling·J M Villalba, P Navas
Mar 23, 2002·The Journal of Eukaryotic Microbiology·Gautam Datta, Tanmoy Bera
Apr 1, 1990·The Journal of Pharmacy and Pharmacology·R K Singh, M A Barrand
Apr 21, 2011·American Journal of Physiology. Endocrinology and Metabolism·Joshua P GrayEmma Heart
Nov 5, 1986·Biochimica Et Biophysica Acta·S Yamashoji, G Kajimoto
Mar 14, 1997·The Journal of Biological Chemistry·B GarnerW Jessup
Mar 23, 1992·Biochimica Et Biophysica Acta·I L SunW P Faulk
May 15, 1991·Biochemical and Biophysical Research Communications·W Toole-SimmsD J Morré
Nov 15, 1990·Biochemical and Biophysical Research Communications·I L SunD J Morré
Feb 26, 1986·Biochemical and Biophysical Research Communications·P NavasF L Crane
Apr 15, 1991·Biochemical and Biophysical Research Communications·I L SunW P Faulk

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.