PMID: 6125076Jan 1, 1982

Properties of alkaline phosphatases from cellular cementum of rat molars

Acta Odontologica Scandinavica
G Granström

Abstract

The characteristics of nonspecific alkaline phosphatase, (APase, EC 3.1.3.1.) measured as beta-glycerophosphatase (GPase, EC 3.1.3.1.), inorganic pyrophosphatase (PPiase, EC 3.6.1.1.) and adenosine triphosphatase (ATPase, EC 3.6.1.3.) were studied in detail of butanol extracts prepared from rat molar cementum. Mg2+ was not absolutely essential to any of the activities, but at low levels was stimulatory in all cases. Higher concentrations were inhibitory. Ca2+ stimulated ATPase activity weakly at low levels, but was slightly inhibitory to the other enzyme activities. All enzyme activities showed nearly identical sensitivities to heat inactivation and to L-p-bromotetramisole and levamisole, which caused nearly complete inhibition. About 10-15% of the ATPase activity was insensitive to L-p-bromotetramisole and levamisole. The data are consistent with the concept that GPase, PPiase and ATPase activities of cementum to a major part stem from one enzyme, namely nonspecific alkaline phosphatase.

References

Sep 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G Granström, A Linde
Apr 1, 1975·The Biochemical Journal·R Felix, H Fleisch
Jan 1, 1973·Scandinavian Journal of Dental Research·H FredénA Linde
May 20, 1974·Biochimica Et Biophysica Acta·R Felix, H Fleisch
Jan 1, 1974·Calcified Tissue Research·B C MagnussonT Arwill
Aug 1, 1972·Archives of Oral Biology·G Granström, A Linde
Sep 1, 1968·The Biochemical Journal·J K Smith, D W Moss
Jan 1, 1981·Calcified Tissue International·G Granström, A Linde
Mar 29, 1960·Annals of the New York Academy of Sciences·M S BURSTONE
Mar 1, 1962·Archives of Oral Biology·A R TEN CATE
Mar 1, 1961·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K NUKI, S L BONTING
Dec 1, 1967·The Biochemical Journal·R H Eaton, D W Moss
Jan 1, 1923·The Biochemical Journal·R Robison

Citations

Mar 8, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Aus Tariq AliNigel J Crowther
Jun 1, 1988·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·C EngströmB Thilander
Jun 1, 1987·Journal of Dental Research·L P Nilsson, G Granström

Related Concepts

Glycerol-2-phosphatase
DNA-dependent ATPase
Alkaline Phosphatase
Dental Cementum
Hydrogen-Ion Concentration
Molar Tooth
Phosphoric Monoester Hydrolases
Pyrophosphatase
August Rats

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