PMID: 7033779Nov 1, 1981Paper

Properties of alpha-hydroxyacid dehydrogenase isozymes from Trypanosoma cruzi

Molecular and Biochemical Parasitology
C E CoronelA Blanco

Abstract

Whole cell extracts of culture epimastigotes of Trypanosoma cruzi (Tulahuén strain) have alpha-hydroxyacid dehydrogenase activity which catalyzes the NAD-linked reaction alpha-ketoacid in equilibrium with alpha-hydroxyacid, with a variety of substrates. Two molecular forms of the enzyme have been separated by means of gel electrophoresis. These isozymes were partially purified by DEAE-cellulose chromatography and ammonium sulfate precipitation. Molecular weights were estimated and some catalytic properties were determined with purified isozymes. The faster migrating fraction (isozyme I) has a molecular weight of 85 500 and showed significant activity against linear 3-5 carbon chain substrates. The lowest Km value was obtained for pyruvate. Isozyme II (MW 60 500) utilizes linear and branched chain substrates with 4-6 carbon atoms. Its highest activity and lowest Km value were recorded with alpha-keto-isocarproate as substrate.

References

May 1, 1977·Archives of Biochemistry and Biophysics·S M HutsonH A Lardy
Feb 1, 1976·The Biochemical Journal·A BlancoE E Montamat
Jul 1, 1968·Archives of Biochemistry and Biophysics·J L Hedrick, A J Smith
Aug 1, 1965·The Journal of Experimental Zoology·C L Markert, I Faulhaber
Jul 1, 1964·The Journal of Experimental Zoology·A BLANCOL KUPCHYK

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Citations

Feb 1, 1984·Molecular and Biochemical Parasitology·C E CoronelA Blanco
Jan 15, 1987·Molecular and Biochemical Parasitology·E E MontamatA Blanco
Jan 1, 1984·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J J Cannata, J J Cazzulo
Jan 1, 1984·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J J Cazzulo
Sep 23, 1997·Molecular and Biochemical Parasitology·A D Uttaro, F R Opperdoes
Jul 1, 1990·The Journal of protozoology·L E RovaiA Blanco
May 9, 2008·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Man Chin ChungLorena Blau
Mar 1, 1983·The Journal of Experimental Zoology·C E CoronelA Blanco
Nov 1, 1983·The Journal of protozoology·A BlancoL E Rovai
May 24, 2007·Journal of Enzyme Inhibition and Medicinal Chemistry·Charmina Aguirre-AlvaradoCarlos Wong
Dec 25, 2009·Journal of Enzyme Inhibition and Medicinal Chemistry·Charmina Aguirre-AlvaradoCarlos Wong
Sep 26, 2003·Journal of Enzyme Inhibition and Medicinal Chemistry·Silvia ElizondoCarlos Wong

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