PMID: 7543928Sep 1, 1995Paper

Properties of AMPA receptors expressed in rat cerebellar granule cell cultures: Ca2+ influx studies

Journal of Neurochemistry
N Hack, R Balázs

Abstract

Cultured cerebellar granule cells become vulnerable to excitatory amino acids, especially to NMDA and kainate, by 9 days in vitro. In the same time, the sensitivity of cells to (RS)-alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionate (AMPA), in terms of AMPA-induced toxicity or 45Ca2+ uptake, was very low. The low AMPA responsiveness was due to receptor desensitization, because agents known to block desensitization, cyclothiazide and the lectins concanavalin A and wheat germ agglutinin, rendered granule cells vulnerable to AMPA and produced a pronounced stimulation of 45Ca2+ accumulation. 45Ca2+ influx was induced specifically by AMPA-receptor stimulation, because it was blocked virtually completely by 2,3-dihydroxy-6-nitro-7-sulfamoylbenzoquinoxaline (NBQX) and the benzodiazepine GYKI 52466 (selective non-NMDA receptor antagonists). Nevertheless, indirect routes activated by cellular responses to AMPA-receptor stimulation contributed significantly to the overall 45Ca2+ influx. These included Ca2+ uptake through NMDA-receptor channels, voltage-sensitive Ca2+ channels, and via Na+/Ca2+ exchange. However, nearly one-fifth of the total 45Ca2+ influx remained unaccounted for and this estimate was similar to 45Ca2+ influx observed...Continue Reading

Citations

Dec 1, 1996·Naunyn-Schmiedeberg's Archives of Pharmacology·G CebersS Liljequist
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·A J HampsonD Wink
Mar 20, 2001·The Journal of Clinical Investigation·E KoustovaA S Basile
Jan 5, 2000·Physiological Reviews·D G Nicholls, S L Budd
Mar 10, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·H S Mogensen, O S Jorgensen
Jan 1, 1997·Brain Research Bulletin·G CebersS Liljequist
Jun 27, 1995·Brain Research. Developmental Brain Research·N J HackR Balázs
Jun 30, 2001·Japanese Journal of Pharmacology·N KönigJ M Exbrayat
May 5, 2007·Molecular and Cellular Neurosciences·M Chiara ManziniCarol A Mason

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