Properties of an inwardly rectifying K(+) channel in the basolateral membrane of mouse TAL

American Journal of Physiology. Renal Physiology
M PaulaisJ Teulon

Abstract

We investigated the properties of K(+) channels in the basolateral membrane of the cortical thick ascending limb (CTAL) using the patch-clamp technique. Approximately 34% of cell-attached patches contained an inwardly rectifying K(+) channel (K(+)-to-Na(+) permeability ratio approximately 22), having an inward conductance (G(in)) of 44 pS and an outward conductance (G(out)) of approximately 10 pS (G(in)/G(out) approximately 4). Channel activity (NP(o)) increased with depolarization. When the cytosolic sides of inside-out patches were exposed to an Mg(2+)-free medium, the channel had a G(in) of 50 pS and was weakly inwardly rectifying (G(in)/G(out) approximately 1). Cytosolic Mg(2+) reduced G(out), yielding a G(in)/G(out) of 3.8 at 1.3 mM Mg(2+). Internal Na(+) also yielded a G(in)/G(out) of 1.6 at 20 mM Na(+). Spermine reduced NP(o) on inside-out membrane patches. Sensitivity to spermine at depolarizing voltages [half-maximal inhibitory concentration (K(i)) = 0.2 microM] was much greater than at hyperpolarizing voltages (K(i) = 26 microM). Half-inactivation by 0.5 microM spermine occurred at a clamp potential of 43 mV, with an effective valence of 1.25. A sigmoid relationship between bath pH and NP(o) of inside-out membrane pat...Continue Reading

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Citations

Dec 25, 2004·Physiological Reviews·Steven C HebertWenhui Wang
Feb 1, 2006·The Journal of General Physiology·Marc PaulaisJacques Teulon
Jun 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Marc PaulaisStephen J Tucker
Feb 18, 2012·American Journal of Physiology. Renal Physiology·Kirk L Hamilton, Daniel C Devor
Nov 15, 2011·Molecular Genetics and Metabolism·Bikash R PattnaikDe-Ann M Pillers
Aug 10, 2004·Current Opinion in Nephrology and Hypertension·WenHui Wang
Jan 10, 2019·American Journal of Physiology. Renal Physiology·Xiao-Tong SuWen-Hui Wang
May 4, 2007·American Journal of Physiology. Renal Physiology·Ruimin GuBaofeng Yang
Mar 28, 2008·American Journal of Physiology. Renal Physiology·Sahran LachhebMarc Paulais
Jan 23, 2003·The Journal of Pharmacology and Experimental Therapeutics·Tong Wang
Apr 3, 2015·American Journal of Physiology. Renal Physiology·Chengbiao ZhangWen-Hui Wang
Dec 29, 2013·American Journal of Physiology. Renal Physiology·Mingxiao WangRuimin Gu
Dec 30, 2014·Physiological Reviews·Francisco V SepúlvedaMaría Isabel Niemeyer
Aug 23, 2008·The Tohoku Journal of Experimental Medicine·Elnur Ilham FarajovYoshiaki Kondo
Aug 9, 2002·American Journal of Physiology. Renal Physiology·Rui-Min Gu, Wen-Hui Wang
Jan 13, 2006·American Journal of Physiology. Renal Physiology·Antoine NissantJacques Teulon
Aug 1, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yipin WuAylin R Rodan

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