PMID: 36834Jan 1, 1978

Properties of beta-glucan synthetase from Saccharomyces cerevisiae

Antonie van Leeuwenhoek
E López-Romero, J Ruiz-Herrera

Abstract

Properties of beta-glucan synthetase from S. cerevisiae were studied. The enzyme exhibited optimal activity at pH 6.7 and 24 C. Km for UDP-glucose was 0.12 mM. Addition of Mg++ or Mn++ stimulated its activity by 60% and 21% respectively. High concentrations of EDTA and hydroxyquinoline were inhibitory. Glucan synthetase was fully active in cell-free extracts. Small concentrations of trypsin or subtilopeptidase A from Bacillus subtilis, caused only a slight increase in glucosyl transferase activity, but larger concentrations destroyed beta-glucan synthetase. Acid proteases were neither stimulatory nor destructive. Thus it seems unlikely that beta-glucan synthetase exists in a zymogen form. Glucan synthetase was unstable. It was inactivated more rapidly at 28 C than at 0 C. The presence of substrate, beta-glucan or the protease inhibitors PMSF, Antipain or Pepstatin A did not protect beta-glucan synthetase from inactivation. Glucan synthetase was not stimulated by addition of cellobiose or beta-glucans. The synthesis of beta-glucans was competitively inhibited by UDP (Ki = 0.45 mM). Glucono-delta-lactone, a known inhibitor of beta-glucosidases was a strong non-competitive inhibitor of beta-glucan synthetase.

References

Jan 1, 1976·Antonie van Leeuwenhoek·E López-Romero, J Ruiz-Herrara
Dec 22, 1977·Biochimica Et Biophysica Acta·E López-Romero, J Ruiz-Herrera
Sep 1, 1975·Journal of General Microbiology·R SentandreuJ R Villanueva
Jul 1, 1976·Archives of Biochemistry and Biophysics·M C Wang, S Bartnicki-Garcia
Aug 1, 1976·Archives of Microbiology·T G VillaJ R Villanueva
Dec 1, 1976·Journal of General Microbiology·J Ruiz-Herrera, S Bartnicki-Garcia
Aug 13, 1974·Biochemistry·P H McVerry, K H Kim
Sep 15, 1973·Biochimica Et Biophysica Acta·V FarkasS Bauer
Sep 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·E Cabib, V Farkas
Nov 6, 1969·Biochemical and Biophysical Research Communications·A Endo, T Misato
Dec 17, 1969·Journal of the American Chemical Society·K IsonoS Suzuki
Sep 1, 1968·The Biochemical Journal·A T Abd-el-Al, H J PHAFF
Sep 22, 1966·Biochemical and Biophysical Research Communications·M C Wang, S Bartnicki-Garcia
Aug 1, 1953·The Biochemical Journal·M DIXON
Jan 1, 1963·Annual Review of Microbiology·H J PHAFF

Related Concepts

Cell-Free System
Drug Stability
Enzyme Activation
Polycose
Glucosyltransferases
Hydrogen-Ion Concentration
Saccharomyces cerevisiae
Uridine Diphosphate Glucose

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