PMID: 7398623May 1, 1980Paper

Properties of biospecific adsorbents, obtained by immobilization of oestradiol 7 alpha-derivatives, for purification of calf-uterine cytosol oestradiol receptor

European Journal of Biochemistry
G RedeuilhH Richard-Foy

Abstract

The properties of three types of adsorbents obtained by coupling oestradiol 7 alpha-derivatives to agarose were compared. The adsorbents examined were: oestradiol 7 alpha-decamethylene-agarose, oestradiol 7 alpha-decamethylene-poly(anayl-lysine)-agarose and oestradiol 7 alpha-trimethylene-poly(alanyl-lysine)-agarose. The following results were obtained. (1) All these adsorbents are stable at 0 degrees C for a least a year when stored in water. In the presence of cytosol they are stable for several hours and are reusable after a simple wash. (2) A new method allowing the calculation of the maximala receptor binding capacity of an absorbent was developed. (3) The geometry of the column and the dynamics of the loading have no influence on the binding capacity of the adsorbents. (4) Binding of the cytosol receptor to the adsorbent depends on whether the receptor had previously been partially purified by heparin-Ultrogel chromatography or treated with low or high salt concentration or trypsin. It was demonstrated that aggregation decreases the binding of the receptor to the adsorbents. (5) A satisfactory recovery of receptor upon elution is possible only with biospecific adsorbents containing low concentrations of coupled steroid (l...Continue Reading

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M MolinariG A Puca
Dec 1, 1973·Analytical Biochemistry·W Schaffner, C Weissmann
Jan 1, 1972·Gynecologic Investigation·C GeynetE E Baulieu
Feb 1, 1973·The Journal of Clinical Endocrinology and Metabolism·M A Binoux, W D Odell
Jun 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·D ToftJ Gorski
Feb 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·E V JensenE R DeSombre
Dec 23, 1969·Biochimica Et Biophysica Acta·E Milgrom, E E Baulieu
Oct 1, 1970·Analytical Biochemistry·T ErdosR Bessada
Dec 1, 1970·European Journal of Biochemistry·M Best-BelpommeT Erdos
Apr 20, 1971·Biochimica Et Biophysica Acta·H Truong, E E Baulieu

Related Concepts

estradiol 7 alpha-decamethylene-sepharose
estradiol 7 alpha-decamethylene-poly(alanyllysine)-sepharose
estradiol 7 alpha-trimethylene-poly(alanyllysine)-sepharose
Adsorption
Bos indicus
Chromatography, Affinity
Cytoplasmic Matrix
SDS-PAGE
Estradiol, (16 alpha,17 beta)-Isomer
Polysaccharides

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