PMID: 6162004Feb 1, 1981Paper

Properties of bovine oligodendroglia isolated by a new procedure using physiologic conditions

Journal of Neurochemistry
M FarooqW T Norton

Abstract

A new class of procedures, previously shown to permit the isolation of pure oligodendroglia from whole rat cerebrum, has been applied with equal or greater success for the bulk isolation of this cell type from bovine white matter. Thus, the generality of this approach has been demonstrated. The bovine preparations have a purity of greater than 90% intact, phase-bright oligodendroglia and are obtained in a yield of 8 x 10(6) cells per gram of white matter. Within 1 day it is possible to obtain a preparation containing 60 mg of protein from a single cell type. These cells show a higher degree of ultrastructural preservation of all cytoplasmic constituents than previously obtained. The values for protein (33 pg/cell), DNA (5.4 pg/cell), and lipid (5-6 pg/cell) are very similar to those obtained with an earlier procedure. The cell lipids are rich in galactolipid, which comprises 20% of the total. The activity of the "myelin-specific" enzyme, 2',3'-cyclic nucleotide 3'-phosphohydrolase (EC 3.1.4.37), is 4.7 mumol/min/mg protein, similar to that obtained previously for isolated oligodendroglia and about 25-40% of that found in myelin. The activity of 5'-nucleotidase (EC 3.1.3.5) in the cells is about 10% of that in myelin or white ma...Continue Reading

References

Mar 1, 1979·Journal of Neurochemistry·T Abe, W T Norton
Jan 1, 1979·Neuropathology and Applied Neurobiology·K Yajima, K Suzuki
Dec 15, 1978·Brain Research·G W KreutzbergP Schubert
Mar 18, 1977·Brain Research·M FarooqW T Norton
Oct 7, 1977·Brain Research·D PleasureT Saida
Apr 1, 1977·Journal of Neurochemistry·K IqbalH M Wisniewski
Jan 1, 1975·Journal of Neuroscience Research·W T NortonG H DeVries
Mar 19, 1971·Brain Research·C S RaineW T Norton
Mar 1, 1972·Journal of Neurochemistry·S E Poduslo, W T Norton
Nov 1, 1973·The Journal of Cell Biology·C R Hopkins, M G Farquhar
Apr 1, 1966·Journal of Neurochemistry·W T Norton, L A Autilio
Aug 1, 1980·Journal of Neurochemistry·W CammerW T Norton
Jun 1, 1976·Neuroscience Letters·N L Banik, M E Smith

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Citations

Jul 1, 1981·Neurochemical Research·G ArientiG Porcellati
Feb 1, 1984·Journal of the Neurological Sciences·L C Doering, S Fedoroff
Apr 16, 1993·Brain Research. Developmental Brain Research·W T Norton, M Farooq
Nov 1, 1986·Journal of Neuroimmunology·E SmyrnisD W Paty
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S SzuchetS Monsma
Dec 1, 1983·Journal of Neuroimmunology·J S PedersenC C Bernard
Apr 1, 1996·Neurochemical Research·W T Norton
Dec 24, 1984·Brain Research·A SuzumuraD H Silberberg
Sep 1, 1984·Journal of Neurochemistry·A Gibson, M J Brammer
Jun 1, 1989·Journal of Neuroscience Research·R K YuR W Ledeen
Jul 30, 2019·Journal of Neurochemistry·Robert K Yu
Jan 1, 1985·Progress in Lipid Research·P S Sastry
Jan 1, 1983·Journal of Neuroscience Research·E J Bradel, F P Prince

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