Properties of cerebral polyribosomes and membranes of the rough endoplasmic reticulum after ethanol dependence in rats

Alcoholism, Clinical and Experimental Research
E W Fleming, S Tewari

Abstract

Effects of physical dependence upon ethanol on the polyribosomal properties and the reconstitution of the rough endoplasmic reticulum (RER) of the brain has been examined. The purified free polyribosomes (polysomes), membrane-bound polysomes, and a fraction of RER membrane that has been stripped of polysomes were isolated from rat brain. RNA yields, amino acid incorporation activities, and electron micrographs established the purity of stripped membranes, but no differences were detected following the ethanol treatment. For the polyribosomal fractions, the stability of the mRNA/ribosomal complex was decreased after ethanol dependence as was the in vitro translation of endogenous mRNA. In the reconstitution reaction, the incubation of membranes from ethanol-treated animals with either source of purified bound polysomes resulted in higher yields of protein than when control membranes were used. The above results suggest that ethanol dependence affects the properties of both the RNA and membrane components of the RER of brain.

References

Mar 1, 1975·Journal of Neurochemistry·E W FlemingE P Noble
Jan 1, 1977·Alcoholism, Clinical and Experimental Research·O A Parsons
Jan 1, 1975·Advances in Experimental Medicine and Biology·S Tewari, E P Noble
Mar 1, 1975·Journal of Neurochemistry·S TewariE P Noble
Jun 20, 1972·Biochimica Et Biophysica Acta·S J PatersonH Schneider
Jan 26, 1973·Biochimica Et Biophysica Acta·J R TrudellE N Cohen
Apr 30, 1973·Annals of the New York Academy of Sciences·E P Noble, S Tewari
Jul 14, 1970·Brain Research·E Glassman, J E Wilson
Oct 14, 1969·Biochimica Et Biophysica Acta·S M Johnson, A D Bangham
Sep 1, 1980·Neurochemical Research·S TewariE W Fleming
Jan 1, 1965·British Medical Bulletin·J H QUASTEL

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Citations

Feb 1, 1987·Neurochemical Research·S TewariP Najarian
Aug 1, 1988·Neurochemical Research·S TewariE W Fleming

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