PMID: 8952953Dec 1, 1996Paper

Properties of chicken lens MIP channels reconstituted into planar lipid bilayers

The Journal of Membrane Biology
E ModestoA C Campos de Carvalho

Abstract

Membrane fractions highly enriched in chicken lens MIP (MIP28) were found to form ion channels when incorporated into planar lipid bilayers. The channels displayed prominent unitary conductances of about 60 and 290 pS in symmetric 150 mm KCl solution and were slightly anion selective. For both depolarizing and hyperpolarizing voltages, voltage sensitivity of the MIP28-induced conductance could be fit by a Boltzmann relation, symmetric around zero mV, with V0 = 18.5 mV, n = 4.5 and gmin/gmax = 0.17. Channel properties were not appreciably altered by pH in the range of 5.8 to 7, although channel incorporation was observed to occur more frequently at lower pH values. Calcium, at millimolar concentrations, decreased the channel mean open time. Partial proteolysis of MIP28 to yield MIP21 did not appreciably affect single-channel conductance or voltage sensitivity of the reconstituted channels. MIP28 was not phosphorylated by cAMP dependent protein kinase (PKA). Although unitary conductance and selectivity of the chicken MIP channel are similar to those reported for the bovine MIP (MIP26), the voltage sensitivity of MIP28 was higher than that of the bovine homologue, and voltage sensitivity of MIP28 was not modulated by treatments pr...Continue Reading

Citations

Jul 9, 1998·Protein Science : a Publication of the Protein Society·A FrogerC Delamarche
Nov 30, 2002·Trends in Pharmacological Sciences·Daniela Boassa, Andrea J Yool
Feb 22, 2012·Molecular Aspects of Medicine·Andrea J Yool, Ewan M Campbell
Sep 29, 2007·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Andrea J Yool
Jun 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Núria LloberasJosep M Grinyó

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