PMID: 41580Dec 7, 1979

Properties of cyclic AMP-dependent protein kinase in normal and goitrous rat thyroid gland

Biochimica Et Biophysica Acta
O HabhabN S Halmi

Abstract

Most of the cyclic AMP-dependent protein kinase activity in propylthiouracil-induced goiters and control rat thyroid glands was found in the soluble fraction. The activity in the particulate fractions was cyclic AMP-independent. Protein kinase activity was 2--3-fold higher in all the subcellular fractions of goitrous tissue than of control tissue. In the presence of Triton X-100, both groups showed a significant increase in kinase activity in all subcellular fractions, and the kinase activity in the particulate fractions could now be slightly stimulated by cyclic AMP. Again, enzyme activity in fractions from goiters was significantly higher than in control tissue. Two major peaks, Types I and II, of soluble cyclic AMP-dependent protein kinase activity could be separated by DEAE-cellulose chromatography. Chronic in vivo stimulation by TSH was associated with a selective increase in Type II isoenzyme activity. Elution and pH profiles, dissociation of subunits with 0.5 M NaCl, and activity ratios (-cyclic AMP/+cyclic AMP) for various substrates for Type II isoenzyme in goitrous and control tissue were similar. The elevated activity in goitrous tissue was manifested by an increase in V for histone, ATP, Mg2+ and cyclic AMP, with no...Continue Reading

References

Apr 1, 1975·Endocrinology·S W Spaulding, G N Burrow
Jan 1, 1975·Annual Review of Biochemistry·C S Rubin, O M Rosen
Jan 1, 1975·Biochimie·M Pavlovic-Hournac, D Delbauffe
Oct 15, 1976·FEBS Letters·D Delbauffe, M Pavlovic-Hournac
Dec 6, 1976·Biochemical and Biophysical Research Communications·C V ByusD H Russell
May 30, 1978·Biochemical and Biophysical Research Communications·R RoskoskiD D Lund
Dec 30, 1971·Annals of the New York Academy of Sciences·G A Robison, E W Sutherland
Feb 1, 1972·Metabolism: Clinical and Experimental·K Yamashita, J B Field
Nov 1, 1972·Endocrinology·S W Spaulding, G N Burrow
Jul 10, 1974·Biochemical and Biophysical Research Communications·S W Spaulding, G N Burrow
Jul 31, 2002·Applied and Environmental Microbiology·John B CliffDavid D Myrold

Related Concepts

Cyclic AMP, (R)-Isomer
Metazoa
Goiter
Hydrogen-Ion Concentration
Alloenzymes
Propylthiouracil
Protein KINASE
Subcellular Fractions
Substrate Specificity
Thyroid Gland

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.