PMID: 6108784Dec 2, 1980Paper

Properties of detergent-solubilized and membranous (Ca2+ + Mg2+)-activated ATPase from sarcoplasmic reticulum as studied by sulfhydryl reactivity and ESR spectroscopy. Effect of protein-protein interactions

Biochimica Et Biophysica Acta
J P AndersenJ V Møller

Abstract

(1) Sulfhydryl reactivity and electron spin resonance spectra of nitroxide maleimide spin labels, covalently attached to sarcoplasmic reticulum ATPase, were examined on both detergent-solubilized and membranous material. Monomeric and oligomeric ATPases were prepared by the use of dodecyloctaethylene glycol monoether as a solubilizing detergent. (2) Immediately after solubilization, the reaction curve of nonomeric ATPase with 5,5'-dithiobis(2-nitrobenzoate) was characterized by positive cooperativity (S-shaped as a function of time). In contrast, the SH reactivity of both oligomeric and membranous ATPases obeyed usual first-order kinetics and could be analyzed in terms of three classes of reactive site. All enzymatically active ATPase preparations responded to addition of ADP with a decrease in SH reactivity. During enzymatic inactivation of monomeric ATPase, the SH-modification rate was dramatically enhanced with loss of cooperative features. Ca2+ removal from the high-affinity sites stimulated SH reactivity before inactivation had taken place. (3) ESR spectroscopy indicated less motional constraints on monomeric than on oligomeric and membranous ATPases. Arrhenius plots of ESR spectral parameters suggest a conformational tran...Continue Reading

References

Jan 3, 1977·European Journal of Biochemistry·Y Dupont
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Citations

May 1, 1995·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·F G de ToledoE N Chini
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J P AndersenJ V Møller
Jun 23, 2004·European Journal of Biochemistry·Yolanda Gutiérrez-MartínFernando Henao
Aug 21, 2013·PloS One·Ana V AntaloaeThomas L-M Sørensen
Jan 1, 1989·Journal of Neurochemistry·S K Yamagata, S M Parsons
Apr 5, 2000·The Journal of Biological Chemistry·P ChampeilM le Maire
Jun 1, 1989·Journal of Muscle Research and Cell Motility·P M Hardwicke, P Huvos
May 24, 2011·Biochemical Society Transactions·Bertrand ArnouMarc le Maire
Mar 28, 1986·Biochemical and Biophysical Research Communications·P OttolenghiJ Jensen
Dec 6, 2005·Biochimica Et Biophysica Acta·Dikran ToroserRajindar S Sohal

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