PMID: 9621Aug 1, 1976

Properties of DNA ligase from uninfected and virus-infected HeLa cells

Nucleic Acids Research
S Spadari

Abstract

HeLa cells contain a high M.W. form of DNA ligase which can be completely converted to a low M.W. form. Stokes radius, frictional ratio, sedimentation coefficient, molecular weight, pH dependence, and heat inactivation rate of the two forms have been studied. The major properties of the two forms of DNA ligase in HeLa cells (in particular molecular weights and pH dependence) resemble those of the "dimer" and "monomer" structures described in cultured human cells (Pedrali, G., Spadari, S., Ciarrocchi, G., Pedrini, M., Falaschi, A. (1973) Eur. J. Biochem., 39 343) . In synchronized HeLa cells, the DNA ligase shows a two fold increase during S phase and parallels the increase in the DNA synthesis rate. DNA ligase increases in parallel with viral DNA synthesis after infection of HeLa cells with vaccinia and Herpes virus but its cofactor requirements and physical properties (including the dimer leads to monomer conversion) are unchanged, suggesting that the newly formed ligase is not virus-coded.

References

Feb 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S Spadari, A Weissbach
Mar 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·U BertazzoniS Spadari
Nov 15, 1973·European Journal of Biochemistry·G C Pedrali NoyA Falaschi
Dec 22, 1972·Biochimica Et Biophysica Acta·U BertazzoniF Campagnari
Mar 1, 1973·Archives of Biochemistry and Biophysics·P BöhlenS Udenfriend
Feb 15, 1973·Journal of Molecular Biology·L M ChangF J Bollum
Aug 6, 1973·Biochemical and Biophysical Research Communications·S Söderhäll, T Lindahl
Sep 13, 1971·European Journal of Biochemistry·S SpadariA Falaschi
Sep 1, 1969·The Journal of Cell Biology·D M BerkowitzM Sporn

Citations

Jan 1, 1979·Pharmacology & Therapeutics. Part B: General & Systematic Pharmacology·S Kit
Sep 1, 1990·Mutation Research·D D LaskoT Lindahl
Nov 25, 1989·Nucleic Acids Research·S M Kerr, G L Smith
Nov 1, 1978·Nucleic Acids Research·M Mezzina, S Nocentini
Jan 15, 1987·European Journal of Biochemistry·M MezzinaA Sarasin
Dec 1, 1977·Virology·M EstebanJ A Holowczak

Related Concepts

HeLa Cells
Hydrogen-Ion Concentration
Bacteriophage Plaque Assay
Protein Conformation
Protein Denaturation
Thymidine Kinase
Buffalopox virus
Herpesvirus 16, Cercopithecine

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

The Tendon Seed Network

Tendons are rich in the extracellular matrix and are abundant throughout the body providing essential roles including structure and mobility. The transcriptome of tendons is being compiled to understand the micro-anatomical functioning of tendons. Discover the latest research pertaining to the Tendon Seed Network here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Incretins

Incretins are metabolic hormones that stimulate a decrease in glucose levels in the blood and they have been implicated in glycemic regulation in the remission phase of type 1 diabetes. Here is the latest research.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Long COVID-19

“Long Covid-19” describes illness in patients who are reporting long-lasting effects of the SARS-CoV-19 infection, often long after they have recovered from acute Covid-19. Ongoing health issues often reported include low exercise tolerance and breathing difficulties, chronic tiredness, and mental health problems such as post-traumatic stress disorder and depression. This feed follows the latest research into Long Covid.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.