PMID: 3215388Nov 1, 1988Paper

Properties of fatty acyl-coenzyme A: estradiol-17 beta acyltransferase in bovine placenta microsomes

Molecular and Cellular Endocrinology
P MartynJ B Adams

Abstract

The properties of the enzyme catalyzing the formation of non-polar derivatives of estradiol-17 beta (E2) esterified to long-chain fatty acids have been investigated in microsomal preparations from bovine placenta cotyledons. A rapid enzyme assay has been developed which involves simple solvent partitioning. The membrane-bound enzyme showed a pH optimum of 5.0 and addition of fatty acyl-coenzymes A (CoAs), such as oleoyl-CoA, palmitoyl-CoA and palmitoleoyl-CoA, increased [3H]E2-fatty acyl ester formation from [3H]E2 by some 7-fold. Linoleoyl-CoA, linolenoyl-CoA and arachidonoyl-CoA were much less effective as acyl donors. Only 17 beta-fatty acyl monoesters were synthesized in each instance. Similar results were obtained with microsomes or mitochondria from bovine endometrium. The apparent Km for E2 employing placenta microsomes was 8.0 +/- 2.2 (SD) microM. Steroids such as testosterone, dehydroepiandrosterone and 5-androstene-3 beta, 17 beta-diol acted as competitive inhibitors (Ki values 79, 46 and 39 microM, respectively). These, and other data to be reported separately, which showed that these steroids were substrates for the enzyme, demonstrate that the latter is not specific for E2. The [3H]E2-fatty acyl ester fractions bio...Continue Reading

References

Jan 1, 1979·Progress in Lipid Research·A A SpectorT L Kaduce
Apr 29, 1987·Biochemical and Biophysical Research Communications·F T Lee, J B Adams
Jun 1, 1986·Journal of Steroid Biochemistry·J B AdamsS Nott
Oct 1, 1983·Steroids·Y J Abul-Hajj, A Nurieddin
Sep 17, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Gary S DorfmanUNKNOWN Translational Research Working Group

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Citations

Mar 1, 1992·Breast Cancer Research and Treatment·J B Adams
Feb 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·C Thériault, F Labrie
Jan 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·Y AkwaD H Jo
Jan 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·R B HochbergJ M Larner
Apr 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·J E ZielinskiR B Hochberg
Nov 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·J B AdamsC E Young
Jan 1, 1990·Annals of the New York Academy of Sciences·R B HochbergJ E Zielinski
Oct 30, 2007·Beilstein Journal of Organic Chemistry·Dominik A Lange, Bernd Goldfuss
Jun 5, 2004·Marine Environmental Research·Gemma JanerCinta Porte
Jun 17, 1998·Endocrine Reviews·R B Hochberg
Aug 30, 2007·The Journal of Clinical Endocrinology and Metabolism·Maija BadeauMatti J Tikkanen

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