Properties of HERG channels stably expressed in HEK 293 cells studied at physiological temperature

Biophysical Journal
Z ZhouC T January

Abstract

We have established stably transfected HEK 293 cell lines expressing high levels of functional human ether-a go-go-related gene (HERG) channels. We used these cells to study biochemical characteristics of HERG protein, and to study electrophysiological and pharmacological properties of HERG channel current at 35 degrees C. HERG-transfected cells expressed an mRNA band at 4.0 kb. Western blot analysis showed two protein bands (155 and 135 kDa) slightly larger than the predicted molecular mass (127 kDa). Treatment with N-glycosidase F converted both bands to smaller molecular mass, suggesting that both are glycosylated, but at different levels. HERG current activated at voltages positive to -50 mV, maximum current was reached with depolarizing steps to -10 mV, and the current amplitude declined at more positive voltages, similar to HERG channel current expressed in other heterologous systems. Current density at 35 degrees C, compared with 23 degrees C, was increased by more than twofold to a maximum of 53.4 +/- 6.5 pA/pF. Activation, inactivation, recovery from inactivation, and deactivation kinetics were rapid at 35 degrees C, and more closely resemble values reported for the rapidly activating delayed rectifier K+ current (I(Kr...Continue Reading

References

Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R Ollo, T Maniatis
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·J W Warmke, B Ganetzky
Dec 15, 1995·Circulation·M W VeldkampL N Bouman
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·M C SanguinettiM T Keating
May 1, 1996·The Journal of General Physiology·P S SpectorM C Sanguinetti
Dec 31, 1997·The American Journal of Physiology·S MohammadC T January
May 24, 1996·Science·M C TrudeauG A Robertson
Mar 3, 1949·The Journal of Physiology·A L HODGKIN, B KATZ
Sep 20, 1943·The Journal of General Physiology·D E Goldman

❮ Previous
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Citations

May 11, 2007·Archives of Pharmacal Research·Su-Hyun JoHan Choe
Dec 20, 2007·Naunyn-Schmiedeberg's Archives of Pharmacology·Torsten ChristUrsula Ravens
Dec 2, 2010·Naunyn-Schmiedeberg's Archives of Pharmacology·Ingo StaudacherDierk Thomas
Apr 13, 2011·The Journal of Membrane Biology·Fumiaki AndoSeiko Kawano
Apr 7, 2005·Cancer Chemotherapy and Pharmacology·Shu-Zhen ChenYong-su Zhen
Nov 19, 2009·Histochemistry and Cell Biology·Rolando CarrisozaLaura I Escobar
Nov 11, 2008·Pflügers Archiv : European journal of physiology·Andreas FeigenspanReto Weiler
Aug 19, 2009·Pflügers Archiv : European journal of physiology·Wiebke HirdesChristiane K Bauer
Nov 20, 2012·Biomedical Microdevices·Shiva A PortonovoJacob J Schmidt
Jun 6, 2007·Journal of Computer-aided Molecular Design·David J Diller, Doug W Hobbs
Oct 12, 2012·Archives of Pharmacal Research·Seung Ho LeeBok Hee Choi
Oct 3, 2013·Advances in Therapy·Guoliang LiChaofeng Sun
Mar 19, 2013·Biosensors & Bioelectronics·Alokik KanwalReginald C Farrow
Jun 14, 2005·Current Opinion in Chemical Biology·Glenn A ReinhartGary A Gintant
Jan 20, 2004·European Journal of Pharmacology·Martin TraebertWilli Suter
Jun 7, 2005·Journal of Pharmacological and Toxicological Methods·Liang Guo, Heather Guthrie
Jun 7, 2005·Journal of Pharmacological and Toxicological Methods·Jian-An YaoPhilip Atterson
Jan 22, 2004·Journal of Molecular and Cellular Cardiology·Zhi SuGary Gintant
Feb 13, 2001·European Journal of Pharmacology·K FinlaysonJ S Kelly
Nov 8, 2001·European Journal of Pharmacology·K FinlaysonJ S Kelly
Oct 3, 2002·European Journal of Pharmacology·Anna P MalykhinaHamid I Akbarali
Dec 20, 2003·Progress in Cardiovascular Diseases·Ian W GlaaserColleen E Clancy
Aug 26, 2000·Progress in Biophysics and Molecular Biology·B S Brown, S P Yu
May 8, 2001·Trends in Pharmacological Sciences·J I VandenbergT J Campbell
Mar 12, 2004·Computer Methods and Programs in Biomedicine·Roger Marrannes, Erik De Prins
Sep 2, 2003·Epilepsy Research·Bengt R DanielssonTorbjörn Tomson
Dec 10, 2013·Chemical Reviews·Claudine Mayer, Yves L Janin
May 5, 2012·Molecular Pharmaceutics·Laura B PetersonBrian S J Blagg
Jul 11, 2000·Allergy·M Taglialatela, L Annunziato
Oct 8, 1999·British Journal of Pharmacology·A G TeschemacherH J Witchel
Aug 22, 2000·British Journal of Pharmacology·H TieT J Campbell
Nov 18, 2000·British Journal of Pharmacology·M TaglialatelaL Annunziato
Oct 7, 2000·Clinical and Experimental Pharmacology & Physiology·H J Witchel, J C Hancox

❮ Previous
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