May 3, 1976

Properties of heterocysts isolated with colloidal silica

Archives of Microbiology
R B Peterson, R H Burris

Abstract

A method is described for the isolation of heterocysts that are virtually free of contaminating cell debris after sonication of aerobically grown Anabaena 7120. Isolated heterocysts reduced acetylene in a light-dependent process in the absence of exogenously provided ATP; heterocysts supplied with ATP and Na2S2O4 reduced acetylene slowly in the dark but still showed a marked light activation. Nitrogenase activity was greatest in fractions containing intact heterocysts. Up to 13% of the activity of the intact filaments was accounted for in the isolated heterocyst preparation. Isolated heterocysts took up O2 in a light-independent process; O2 uptake with added NADP+ was enhanced by pyruvate, isocitrate and intermediates of the oxidative pentose pathway.

  • References21
  • Citations10

Mentioned in this Paper

Sonication
Light
Ultracentrifugation
NADP
Oxygen Consumption
Diazotrophy
Cell Separation
Hydrocolloids
Vanadium Nitrogenase
Cyanobacteria

About this Paper

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