Jun 7, 1976

Properties of highly purified lysyl oxidase from embryonic chick cartilage

Biochimica Et Biophysica Acta
F L Stassen

Abstract

Lysyl oxidase the enzyme which oxidately deaminates lysine residues in collagen and elastin, was purified from embryonic chick cartialge by employing an affinity column of lathyritic rat skin collagen coupled to Sepharose, followed by separation on DEAE-cellulose. An enzyme preparation was obtained which was pure as shown by polyacrylamide gel electrophoresis. The specific activity was 1800-fold higher than that of the original extract. The pure enzyme utilized both collagen and elastin substrate. Furthermore, the ratios of enzyme activity with elastin substrate versus that with collagen substrate were the same at all stages of purity. Only one protein band was found after polyacrylamide gel electrophoresis of the pure lysyl oxidase in sodium dodecyl sulfate and mercaptoethanol. The molecular weight was estimated to be 28000. It was found that the enzyme contained a large number of cysteine and tyrosine residues. Evidence was obtained for molecular heterogeneity of lysyl oxidase. The enzyme eluted from DEAE-cellulsoe in at least four distinct regions. When the peaks were rechromatographed separately, they eluted at salt concentrations similar to those of the original chromatogram. However, the substrate specificity and the elec...Continue Reading

  • References17
  • Citations25

References

  • References17
  • Citations25

Citations

Mentioned in this Paper

Embryo
ELN gene
Polyacrylamide Gels
LOX gene
2-Mercaptoethanol
SDS-PAGE
Protein-Lysine 6-Oxidase
Chromatography, DEAE-Cellulose
Lysine
Zyderm

About this Paper

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