PMID: 3768417Nov 19, 1986Paper

Properties of liver retinyl ester hydrolase in young pigs

Biochimica Et Biophysica Acta
D A Cooper, J A Olson

Abstract

A new sensitive method for the assay of retinyl ester hydrolase in vitro was developed and applied to liver homogenates of 18 young pigs with depleted-to-adequate liver vitamin A reserves. Radioactive substrate was not required, because the formation of retinol could be adequately quantitated by reversed-phase high-performance liquid chromatography. Optimal hydrolase activity was observed with 500 microM retinyl palmitate, 100 mM 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate, and 2 mg/ml Triton X-100 at pH 8.0. The relative rates of hydrolysis of six different retinyl esters by liver homogenate were: retinyl linolenate (100%), myristate (99%), palmitate (47%), oleate (38%), linoleate (31%), and stearate (29%). The enzyme was found primarily in the membrane-containing fractions of liver (59 +/- 3%, S.E.) and kidney (76 +/- 3%), with considerably lower overall activity in kidney (57-375 nmol/h per g of tissue) than in liver (394-1040 nmol/h per g). Retinyl ester hydrolase activity in these pigs was independent of serum retinol values, which ranged from 3 to 24 micrograms/dl, and of liver vitamin A concentrations from 0 to 32 micrograms/g. Pig liver retinyl ester hydrolase differs from the rat liver enzyme in its subst...Continue Reading

References

Dec 1, 1979·Archives of Biochemistry and Biophysics·C C Chen, J Heller
Nov 1, 1983·The Journal of Nutrition·J A Olson, D Gunning
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R BlomhoffK R Norum
Apr 1, 1981·Archives of Biochemistry and Biophysics·C C ChenJ Horwitz

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Citations

Jan 1, 1992·Brain Research Bulletin·A T Tsin, D W Malsbury
Feb 11, 2003·The British Journal of Nutrition·Min ChenTapan K Basu
Jan 1, 1987·Drug Metabolism Reviews·F J Leinweber
Sep 1, 1992·Current Eye Research·D L Bernal, J L Ubels
Feb 9, 2005·The American Journal of Clinical Nutrition·Ashley R Valentine, Sherry A Tanumihardjo
Nov 21, 2020·Advances in Nutrition·Bryan M GannonSherry A Tanumihardjo
Oct 13, 1993·Biochimica Et Biophysica Acta·E H Harrison
Oct 22, 1990·Biochimica Et Biophysica Acta·M MercierV Azais-Braesco
May 13, 1994·Biochimica Et Biophysica Acta·M MercierV Azais-Braesco
Feb 15, 1989·Archives of Biochemistry and Biophysics·H FriedmanT J Layden
Feb 1, 1988·Archives of Biochemistry and Biophysics·D A Cooper, J A Olson

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